Tachibana Tetsuya, Hiramatsu Kohzy, Furuse Mitsuhiro, Hasegawa Shin, Yoshizawa Fumiaki, Sugahara Kunio
United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Comp Biochem Physiol A Mol Integr Physiol. 2005 Feb;140(2):203-7. doi: 10.1016/j.cbpb.2005.01.001.
Glucagon-like peptide-1 (GLP-1), structurally similar to glucagon, synthesized from the precursor proglucagon, is a well known anorexigenic peptide in the brain of several animal species. However, there are no previous reports concerning GLP-1-containing neurons in the chick brain. The aim of the present study was to investigate the distribution of proglucagon mRNA and GLP-1-immunoreactive (GLI) perikarya in various regions of the chick brain. We detected proglucagon mRNA in the brainstem, and to a lesser extent in the telencephalon. In the brainstem, a study using immunohistochemistry revealed that GLI perikarya were present in the nucleus motorius nervi facialis pars dosalis, nucleus motoris dorsalis nervi vagi and nucleus tractus solitarii. Furthermore, we found that proglucagon mRNA expression in the brainstem decreased after 24 h fasting. The present findings support the idea that endogenous GLP-1 is involved in feeding behavior of chicks.
胰高血糖素样肽-1（GLP-1）结构上与胰高血糖素相似，由前胰高血糖素原合成，是几种动物脑中一种著名的厌食肽。然而，以前没有关于鸡脑中含GLP-1神经元的报道。本研究的目的是调查鸡脑不同区域前胰高血糖素mRNA和GLP-1免疫反应性（GLI）核周体的分布。我们在脑干中检测到前胰高血糖素mRNA，在端脑中检测到的量较少。在脑干中，一项免疫组织化学研究表明，GLI核周体存在于面神经运动核背侧部、迷走神经背运动核和孤束核中。此外，我们发现禁食24小时后脑干中的前胰高血糖素mRNA表达下降。目前的研究结果支持内源性GLP-1参与雏鸡摄食行为的观点。
