利用超声引导下细针穿刺细胞学检查对多发性硬化症患者颈部淋巴结中的髓磷脂蛋白进行体内检测。

In vivo detection of myelin proteins in cervical lymph nodes of MS patients using ultrasound-guided fine-needle aspiration cytology.

作者信息

Fabriek Babs O, Zwemmer Jack N P, Teunissen Charlotte E, Dijkstra Christine D, Polman Chris H, Laman Jon D, Castelijns Jonas A

机构信息

Department of Molecular Cell Biology and Immunology, VU Medical Center, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands.

出版信息

J Neuroimmunol. 2005 Apr;161(1-2):190-4. doi: 10.1016/j.jneuroim.2004.12.018.

DOI:10.1016/j.jneuroim.2004.12.018

PMID:15748959

Abstract

Cervical lymph nodes (CLN) have been described to be the first lymphoid draining site of the brain. In this study we used ultrasound guided fine needle aspiration cytology (USgFNAC) to obtain cells, in vivo, from non-enlarged CLN of multiple sclerosis (MS) patients and HCs (HC), and investigated whether myelin proteins could be detected. Macrophages containing myelin basic protein (MBP) and proteolipid protein (PLP) were found in CLN of all MS patients, whereas these could only be detected in a minority of HC. This novel approach allows investigation of the first draining site of the brain for in vivo analysis of the immune regulation of MS.

摘要

颈淋巴结（CLN）被认为是大脑的首个淋巴引流部位。在本研究中，我们使用超声引导下细针穿刺细胞学检查（USgFNAC）从多发性硬化症（MS）患者和健康对照者（HC）的未肿大颈淋巴结中获取体内细胞，并研究是否能检测到髓磷脂蛋白。在所有MS患者的颈淋巴结中均发现了含有髓磷脂碱性蛋白（MBP）和蛋白脂蛋白（PLP）的巨噬细胞，而这些仅在少数健康对照者中能检测到。这种新方法使得对大脑首个引流部位进行体内分析以研究MS的免疫调节成为可能。