Veneault-Fourrey Claire, Parisot Denise, Gourgues Mathieu, Laugé Richard, Lebrun Marc-Henri, Langin Thierry
UMR CNRS/UPS 8618, Laboratoire de Phytopathologie Moléculaire, Institut de Biotechnologie des Plantes, Bât. 630, Université ParisXI, 91405 Orsay cedex, France.
Fungal Genet Biol. 2005 Apr;42(4):306-18. doi: 10.1016/j.fgb.2005.01.009.
Conservation of the molecular mechanisms controlling appressorium-mediated penetration during evolution was assessed through a functional study of the ClPLS1 gene from Colletotrichum lindemuthianum orthologous to the MgPLS1 from Magnaporthe grisea, involved in penetration peg development. These two plant-pathogenic Pyrenomycetes differentiate appressoria to penetrate into plant tissues. We showed that ClPLS1 is a functional homologue of MgPLS1 in M. grisea. Loss of ClPLS1 function had no effect on vegetative growth, conidiation or on appressorium differentiation and maturation. However, Clpls1::hph mutants are non-pathogenic on either intact or wounded bean leaves, as a result of a defect in the formation and/or positioning of the penetration pore and consequently in the formation of the penetration peg. These observations suggest that the fungal tetraspanins control a conserved appressorial function that could be required for the correct localization of the site where the penetration peg emerges.
通过对菜豆炭疽菌（Colletotrichum lindemuthianum）中与稻瘟病菌（Magnaporthe grisea）的MgPLS1直系同源的ClPLS1基因进行功能研究，评估了进化过程中控制附着胞介导的穿透作用的分子机制的保守性，该基因参与穿透钉的形成。这两种植物病原性核菌纲真菌都分化出附着胞以侵入植物组织。我们证明ClPLS1是稻瘟病菌中MgPLS1的功能同源物。ClPLS1功能的丧失对营养生长、分生孢子形成或附着胞的分化及成熟没有影响。然而，由于穿透孔的形成和/或定位存在缺陷，进而导致穿透钉形成缺陷，Clpls1::hph突变体在完整或受伤的菜豆叶片上均无致病性。这些观察结果表明，真菌四跨膜蛋白控制着一种保守的附着胞功能，该功能可能是穿透钉出现部位正确定位所必需的。
