Down Michelle, Power Maryanne, Smith Shirley I, Ralston Kylie, Spanevello Mark, Burns Gordon F, Boyd Andrew W
Leukaemia Foundation Laboratory, The Queensland Institute of Medical Research, 300 Herston Road, Herston, Qld 4029, Australia.
Gene Expr Patterns. 2005 Apr;5(4):483-90. doi: 10.1016/j.modgep.2004.12.005.
The cadherin superfamily members play an important role in mediating cell-cell contact and adhesion (Takeichi, M., 1991. Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251, 1451-1455). A distinct subfamily, neither belonging to the classical or protocadherins includes Fat, the largest member of the cadherin super-family. Fat was originally identified in Drosophila. Subsequently, orthologues of Fat have been described in man (Dunne, J., Hanby, A. M., Poulsom, R., Jones, T. A., Sheer, D., Chin, W. G., Da, S. M., Zhao, Q., Beverley, P. C., Owen, M. J., 1995. Molecular cloning and tissue expression of FAT, the human homologue of the Drosophila fat gene that is located on chromosome 4q34-q35 and encodes a putative adhesion molecule. Genomics 30, 207-223), rat (Ponassi, M., Jacques, T. S., Ciani, L., ffrench, C. C., 1999. Expression of the rat homologue of the Drosophila fat tumour suppressor gene. Mech. Dev. 80, 207-212) and mouse (Cox, B., Hadjantonakis, A. K., Collins, J. E., Magee, A. I., 2000. Cloning and expression throughout mouse development of mfat1, a homologue of the Drosophila tumour suppressor gene fat [In Process Citation]. Dev. Dyn. 217, 233-240). In Drosophila, Fat has been shown to play an important role in both planar cell polarity and cell boundary formation during development. In this study we describe the characterization of zebrafish Fat, the first non-mammalian, vertebrate Fat homologue to be identified. The Fat protein has 64% amino acid identity and 80% similarity to human FAT and an identical domain structure to other vertebrate Fat proteins. During embryogenesis fat mRNA is expressed in the developing brain, specialised epithelial surfaces the notochord, ears, eyes and digestive tract, a pattern similar but distinct to that found in mammals.
钙黏蛋白超家族成员在介导细胞间接触和黏附中发挥着重要作用（竹内守，1991年。钙黏蛋白细胞黏附受体作为一种形态发生调节因子。《科学》251卷，1451 - 1455页）。一个独特的亚家族，既不属于经典钙黏蛋白也不属于原钙黏蛋白，其中包括钙黏蛋白超家族中最大的成员Fat。Fat最初是在果蝇中被鉴定出来的。随后，在人类（邓恩，J.，汉比，A. M.，波尔索姆，R.，琼斯，T. A.，希尔，D.，秦，W. G.，达，S. M.，赵，Q.，贝弗利，P. C.，欧文，M. J.，1995年。位于4q34 - q35染色体上、编码一种假定黏附分子的果蝇fat基因的人类同源物FAT的分子克隆及组织表达。《基因组学》30卷，207 - 223页）、大鼠（波纳西，M.，雅克，T. S.，恰尼，L.，弗伦奇，C. C.，1999年。果蝇脂肪肿瘤抑制基因的大鼠同源物的表达。《机制与发育》80卷，207 - 212页）和小鼠（考克斯，B.，哈吉安托纳基斯，A. K.，柯林斯，J. E.，马吉，A. I.，2000年。果蝇肿瘤抑制基因fat的同源物mfat1在小鼠整个发育过程中的克隆与表达[正在引用]。《发育动力学》217卷，233 - 240页）中都发现了Fat的同源物。在果蝇中，已表明Fat在发育过程中的平面细胞极性和细胞边界形成中都发挥着重要作用。在本研究中，我们描述了斑马鱼Fat的特性，它是首个被鉴定出来的非哺乳动物脊椎动物Fat同源物。Fat蛋白与人类FAT有64%的氨基酸同一性和80%的相似性，并且与其他脊椎动物的Fat蛋白具有相同的结构域结构。在胚胎发育过程中，fat mRNA在发育中的大脑、特殊上皮表面、脊索、耳朵、眼睛和消化道中表达，这种表达模式与在哺乳动物中发现的相似但又有所不同。
