Zhang Hongjie, Shinmyo Yohei, Mito Taro, Miyawaki Katsuyuki, Sarashina Isao, Ohuchi Hideyo, Noji Sumihare
Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, 2-1 Minami-Jyosanjima-cho, Tokushima City 770-8506, Japan.
Gene Expr Patterns. 2005 Apr;5(4):491-502. doi: 10.1016/j.modgep.2004.12.006.
We have studied embryogenesis of the two-spotted cricket Gryllus bimaculatus as an example of a hemimetabolous, intermediate germ insect, which is a phylogenetically basal insect and may retain primitive features. We observed expression patterns of the orthologs of the Drosophila homeotic genes, Sex combs reduced (Scr), Antennapedia (Antp), Ultrabithorax (Ubx) and abdominal-A (abd-A) during embryogenesis and compared the expression patterns of these genes with the more basal thysanuran insect, Thermobia domestica (the firebrat), and the derived higher dipteran insect, Drosophila melanogaster. Although Scr is expressed commonly in the presumptive posterior maxillary and labial segment in all three insects, the thoracic expression domains vary. Antp is expressed similarly in the three thoracic segments, the limbs, and the anterior abdominal region among these three insects. The early Antp expression in the firebrat and cricket obeys a segmental register in all three thoracic segments, while in Drosophila its initial expression appears in parasegments 4 and 6. Ubx is expressed in the metathoracic (T3) and abdominal segments similarly in the three insects, whereas the expression pattern in the T3 leg differs among them. abd-A is expressed in the posterior compartment of the first abdominal segment and the remaining abdominal segments in all three insects, although its posterior border varies among them.
我们以双斑蟋（Gryllus bimaculatus）为例研究了渐变态、中胚层昆虫的胚胎发生，双斑蟋是一种系统发育上较为原始的昆虫，可能保留了原始特征。我们观察了果蝇同源异形基因Sex combs reduced（Scr）、触角足（Antp）、超双胸（Ubx）和腹部A（abd-A）直系同源基因在胚胎发生过程中的表达模式，并将这些基因的表达模式与更原始的缨尾目昆虫嗜卷书虱（Thermobia domestica，即衣鱼）以及进化程度更高的双翅目昆虫黑腹果蝇进行了比较。尽管Scr在所有这三种昆虫的假定后上颌和唇节中均有普遍表达，但其在胸部的表达区域有所不同。Antp在这三种昆虫的三个胸节、附肢和腹部前部区域的表达相似。衣鱼和蟋蟀中Antp的早期表达在所有三个胸节中都遵循节段性定位，而在果蝇中其初始表达出现在副节4和6。Ubx在这三种昆虫的后胸（T3）和腹部节段中的表达相似，但其在T3腿中的表达模式在它们之间存在差异。abd-A在所有这三种昆虫的第一腹节的后区室和其余腹节中均有表达，尽管其后边界在它们之间有所不同。
