一种易于制备的无细胞百日咳疫苗的特性鉴定与解毒。百日咳毒素A原体的抗原作用。

Characterization and detoxification of an easily prepared acellular pertussis vaccine. Antigenic role of the A protomer of pertussis toxin.

作者信息

García-Sáinz J A, Romero-Avila M T, Ruíz-Arriaga A, Ruíz-Puente J, Agundis C, Ortiz V, Isibasi A

机构信息

Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, DF.

出版信息

Vaccine. 1992;10(5):341-4. doi: 10.1016/0264-410x(92)90375-t.

DOI:10.1016/0264-410x(92)90375-t

PMID:1574919

Abstract

An acellular pertussis vaccine was prepared from pertussis vaccine concentrates by eliminating the cells by centrifugation and subsequent acid precipitation. SDS-PAGE analysis indicated the presence of 17 clearly defined bands including the subunits of pertussis toxin. The acellular preparation was detoxified with glutaraldehyde and lost its undesirable effects (leukocytosis-promoting activity, histamine-sensitizing activity and decrease in weight gain) but retained a very good protective activity (PD50 approximately 2 micrograms protein/mouse). Sera from mice immunized with this preparation indicated an antibody response against the A protomer of pertussis toxin.

摘要

通过离心和随后的酸沉淀去除细胞，从百日咳疫苗浓缩物中制备了一种无细胞百日咳疫苗。SDS-PAGE分析表明存在17条清晰定义的条带，包括百日咳毒素的亚基。用戊二醛对无细胞制剂进行解毒，消除了其不良作用（促白细胞增多活性、组胺致敏活性和体重增加减少），但保留了很好的保护活性（PD50约为2微克蛋白质/小鼠）。用该制剂免疫的小鼠血清显示出针对百日咳毒素A原体的抗体反应。