三氯生/共聚物/氟化钠牙膏和市售口气清新牙膏对产硫化氢细菌的临床疗效。

Clinical efficacy of a triclosan/copolymer/NaF dentifrice and a commercially available breath-freshening dentifrice on hydrogen sulfide-forming bacteria.

作者信息

Vazquez J, Pilch S, Williams M I, Cummins D

机构信息

Oral Care Advanced Technology, Colgate-Palmolive Company Technology Center, Piscataway, NJ 08854-1343, USA.

出版信息

Oral Dis. 2005;11 Suppl 1:64-6. doi: 10.1111/j.1601-0825.2005.01095.x.

DOI:10.1111/j.1601-0825.2005.01095.x

PMID:15752103

Abstract

OBJECTIVE

The objective of this randomized, cross-over clinical study was to compare the 4-h and overnight effects of a triclosan/copolymer/fluoride (TCF-AF) dentifrice and a commercially available breath freshening (CBF) dentifrice containing fluoride for their ability to control H(2)S-forming bacteria.

METHODS

Following a 1 week washout period of brushing with a regular fluoride dentifrice, subjects reported to the clinical site without performing oral hygiene, eating or drinking in preparation for baseline saliva sampling. After providing a baseline saliva sample, subjects were randomly assigned a test dentifrice and instructed to brush their teeth for 1 min, twice a day for 1 week. On the morning of the eighth day, subjects returned to the test site having refrained from oral hygiene, eating and drinking for overnight sampling. Subjects then brushed for 1 min with the assigned dentifrice, and returned for 2 and 4 h postbrushing evaluations. Following a second 1 week washout, subjects repeated the same regimen, but now using the other dentifrice. Oral micro-flora samples were collected by subjects rinsing with 10 ml of sterile water for 10 s. Each collected sample was serially diluted and plated in duplicate onto lead acetate agar. After incubating for 72 h, dark colonies were counted, expressed as log colony-forming units/ml, and reductions from baseline were calculated.

RESULTS

Compared with baseline, the TCF-AF dentifrice reduced H2S-forming bacteria 0.82, 0.80 and 0.22 log units at the 2 and 4 h and overnight time points compared with 0.60, 0.43 and 0.07 log units for the CBF dentifrice. The TCFAF dentifrice was statistically significantly better (P < 0.05) than the CBF dentifrice at 4 h and overnight after brushing the teeth.

CONCLUSION

The results of this randomized study indicate that the TCF-AF dentifrice is effective in controlling H2S-producing bacteria.

摘要

目的

本随机交叉临床研究的目的是比较三氯生/共聚物/氟化物（TCF-AF）牙膏和市售含氟口气清新（CBF）牙膏在控制产H₂S细菌方面的4小时和过夜效果。

方法

在用普通含氟牙膏刷牙1周的洗脱期后，受试者前往临床研究地点，在此期间不进行口腔卫生清洁、饮食，为采集基线唾液样本做准备。在提供基线唾液样本后，受试者被随机分配一种测试牙膏，并被指示每天刷牙两次，每次1分钟，持续1周。在第8天上午，受试者返回测试地点，在此期间不进行口腔卫生清洁、饮食，以便进行过夜样本采集。然后受试者用分配的牙膏刷牙1分钟，并在刷牙后2小时和4小时返回进行评估。在第二个1周洗脱期后，受试者重复相同方案，但使用另一种牙膏。受试者用10毫升无菌水漱口10秒以收集口腔微生物样本。每个收集的样本进行系列稀释，并一式两份接种到醋酸铅琼脂平板上。培养72小时后，计数黑色菌落，以每毫升菌落形成单位的对数表示，并计算相对于基线的减少量。