Selvakumar Sahithya, Singh Shubhi, Swaminathan Priya
Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology, 603203, Kattankulathur, India.
Int Microbiol. 2025 May;28(Suppl 1):111-118. doi: 10.1007/s10123-024-00537-3. Epub 2024 May 20.
Antimicrobial resistance in bacteria is a global threat that can make antibacterial treatments ineffective. One well-known method of antibiotic resistance and a common defensive mechanism in many harmful bacteria is the synthesis of endogenous hydrogen sulfide (HS) in bacteria. In this study, soil bacteria were screened using the lead acetate agar test and the triple sugar iron test to determine that they were non-endogenous HS producers. This was further validated by full genome analysis of the identified organism against the gene sequences of HS-producing genes. Antibacterial resistance of the bacteria was phenotypically analyzed using the Kirby-Bauer disk diffusion method. Then, the effect of exogenous HS on the antibiotic-resistant bacteria was checked in sodium sulfide, leading to antibiotic re-sensitization.
细菌中的抗菌耐药性是一种全球性威胁，可使抗菌治疗无效。抗生素耐药性的一种众所周知的方法以及许多有害细菌中的常见防御机制是细菌内源性硫化氢（HS）的合成。在本研究中，使用醋酸铅琼脂试验和三糖铁试验对土壤细菌进行筛选，以确定它们不是内源性HS产生菌。通过对已鉴定生物体的全基因组与HS产生基因的基因序列进行分析，进一步验证了这一点。使用 Kirby-Bauer 纸片扩散法对抗菌细菌的耐药性进行了表型分析。然后，在硫化钠中检测外源性HS对耐药细菌的影响，从而导致抗生素重新敏感。
