Dutton T J, Sinkins S P
Vector Research Group, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.
Med Vet Entomol. 2005 Mar;19(1):60-5. doi: 10.1111/j.0269-283X.2005.00557.x.
The mosquito Aedes pseudoscutellaris (Theobald), a member of the Aedes (Stegomyia) scutellaris complex (Diptera: Culicidae), is an important vector of subperiodic Wuchereria bancrofti (Cobbold) (Spirurida: Onchocercidae), causing human lymphatic filariasis, on South Pacific islands. Maternal inheritance of filarial susceptibility in the complex has previously been asserted, and larval tetracycline treatment reduced susceptibility; the maternally inherited Wolbachia in these mosquitoes were suggested to be responsible. To investigate the relationship of these two factors, we eliminated Wolbachia from a strain of Ae. pseudoscutellaris by tetracycline treatment, and tested filarial susceptibility of the adult female mosquitoes using Brugia pahangi (Edeson & Buckley). Filarial susceptibility was not significantly different in Wolbachia-free and infected lines of Ae. pseudoscutellaris, suggesting that the Wolbachia in these mosquitoes do not influence vector competence. Crosses between Wolbachia-infected males and uninfected females of Ae. pseudoscutellaris showed cytoplasmic incompatibility (CI), i.e. no eggs hatched, unaffected by larval crowding or restricted nutrient availability, whereas these factors are known to affect CI in Drosophila simulans. Reciprocal crosses between Ae. pseudoscutellaris and Ae. katherinensis Woodhill produced no progeny, even when both parents were Wolbachia-free, suggesting that nuclear factors are responsible for this interspecific sterility.
伪盾伊蚊（Theobald）是盾形伊蚊（Stegomyia）复合组（双翅目：蚊科）的成员，是南太平洋岛屿上引起人类淋巴丝虫病的亚周期型班氏吴策线虫（Cobbold）（旋尾目：盘尾丝虫科）的重要传播媒介。此前有人断言该复合组中丝虫易感性存在母系遗传，幼虫用四环素处理后易感性降低；有人认为这些蚊子中母系遗传的沃尔巴克氏体是原因所在。为了研究这两个因素之间的关系，我们通过四环素处理从一株伪盾伊蚊中消除了沃尔巴克氏体，并用帕氏布鲁线虫（Edeson & Buckley）对成年雌蚊的丝虫易感性进行了测试。在无沃尔巴克氏体和感染沃尔巴克氏体的伪盾伊蚊品系中，丝虫易感性没有显著差异，这表明这些蚊子中的沃尔巴克氏体不会影响传播能力。伪盾伊蚊感染沃尔巴克氏体的雄蚊与未感染的雌蚊杂交表现出细胞质不亲和（CI），即没有卵孵化，不受幼虫拥挤或营养供应受限的影响，而这些因素已知会影响拟果蝇的细胞质不亲和。伪盾伊蚊与凯瑟琳伊蚊（Woodhill）的正反交均未产生后代，即使双亲均无沃尔巴克氏体，这表明核因子是造成这种种间不育的原因。
