球形链霉菌新霉素生物合成中novWUS的功能表征
Functional characterizations of novWUS involved in novobiocin biosynthesis from Streptomyces spheroides.
作者信息
Thuy Ta Thi Thu, Lee Hei Chan, Kim Chun-Gyu, Heide Lutz, Sohng Jae Kyung
机构信息
Institute of Biomolecule Reconstruction (iBR), Department of Chemistry, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Republic of Korea.
出版信息
Arch Biochem Biophys. 2005 Apr 1;436(1):161-7. doi: 10.1016/j.abb.2005.01.012.
NovW, novU, and novS gene products represent dTDP-4-keto-6-deoxy-D-glucose 3,5 epimarase, C-methyltransferase and dTDP-glucose-4-ketoreductase involved in noviose biosynthetic pathway, respectively. We have expressed three genes to elucidate the functions of NovW, NovU, and NovS in Escherichia coli. NovW and NovU catalyze the formation of dTDP-4-keto-6-deoxy-5-C-methyl-L-lyxo-hexose from dTDP-4-keto-6-deoxy-D-glucose. NovS reduces the product formed from the reaction of NovW with dTDP-4-keto-6-deoxy-D-glucose in the presence of NADH to result in dTDP-l-rhamnose. Furthermore, a pathway for the biosynthesis of noviose is proposed.
NovW、novU和novS基因产物分别代表参与新糖生物合成途径的dTDP-4-酮-6-脱氧-D-葡萄糖3,5-差向异构酶、C-甲基转移酶和dTDP-葡萄糖-4-酮还原酶。我们已表达这三个基因以阐明NovW、NovU和NovS在大肠杆菌中的功能。NovW和NovU催化由dTDP-4-酮-6-脱氧-D-葡萄糖形成dTDP-4-酮-6-脱氧-5-C-甲基-L-来苏糖。在NADH存在的情况下，NovS将NovW与dTDP-4-酮-6-脱氧-D-葡萄糖反应形成的产物还原，生成dTDP-L-鼠李糖。此外，还提出了一条新糖生物合成途径。
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