一条合成 dTDP-6-脱氧-l-塔洛糖和 dTDP-L-鼠李糖的新途径：泰国伯克霍尔德氏菌中的 dTDP-L-鼠李糖 4-差向异构酶。
A new route to dTDP-6-deoxy-l-talose and dTDP-L-rhamnose: dTDP-L-rhamnose 4-epimerase in Burkholderia thailandensis.
机构信息
Department of Biological Science, Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Republic of Korea.
出版信息
Bioorg Med Chem Lett. 2011 Jul 1;21(13):3914-7. doi: 10.1016/j.bmcl.2011.05.030. Epub 2011 May 15.
dTDP-L-rhamnose (dTDP-Rha)-synthesizing dTDP-6-deoxy-L-lyxo-4-hexulose reductase (4-KR) and dTDP-Rha 4-epimerase were characterized from Burkholderia thailandensis E264 by utilizing rmlD(Bth) (BTH_I1472) and wbiB(Bth) (BTH_I1476), respectively. Incubation of the recombinant WbiB(Bth) with RmlA/RmlB/RmlC/Tal, which has previously been shown to generate dTDP-6-deoxy-L-talose (dTDP-6dTal) from α-D-glucose-1-phosphate, dTTP, and NADPH, produced dTDP-Rha. (1)H NMR measurements confirmed that both RmlA/RmlB/RmlC/Tal/WbiB(Bth) and RmlA/RmlB/RmlC/RmlD produced dTDP-Rha. WbiB(Bth) alone produced dTDP-Rha when incubated with dTDP-6dTal. This is the first report to demonstrate epimerase activity interconverting between dTDP-Rha and dTDP-6dTal.
来自泰国伯克霍尔德氏菌 E264 的 dTDP-L-鼠李糖（dTDP-Rha）合成酶 dTDP-6-脱氧-L-赤藓酮-4-己糖还原酶（4-KR）和 dTDP-Rha 4-差向异构酶，分别通过利用 rmlD（Bth）（BTH_I1472）和 wbiB（Bth）（BTH_I1476）来进行鉴定。重组 WbiB（Bth）与 RmlA/RmlB/RmlC/Tal 孵育，此前已经表明该酶能够从α-D-葡萄糖-1-磷酸、dTTP 和 NADPH 生成 dTDP-6-脱氧-L-塔洛糖（dTDP-6dTal），生成 dTDP-Rha。（1）H NMR 测量证实，RmlA/RmlB/RmlC/Tal/WbiB（Bth）和 RmlA/RmlB/RmlC/RmlD 都能产生 dTDP-Rha。当与 dTDP-6dTal 孵育时，WbiB（Bth）本身就能产生 dTDP-Rha。这是首次报道证明差向异构酶活性能够在 dTDP-Rha 和 dTDP-6dTal 之间相互转化。
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