马铃薯-短柄茄染色体附加/代换系的创制与鉴定

Development and characterization of potato-Solanum brevidens chromosomal addition/substitution lines.

作者信息

Dong F, Tek A L, Frasca A B L, McGrath J M, Wielgus S M, Helgeson J P, Jiang J

机构信息

Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA.

出版信息

Cytogenet Genome Res. 2005;109(1-3):368-72. doi: 10.1159/000082421.

DOI:10.1159/000082421

PMID:15753598

Abstract

Solanum brevidens is a wild diploid potato species possessing high levels of resistances to several major potato diseases. We previously developed fertile somatic hybrids between S. brevidens and the cultivated potato (Solanum tuberosum) in order to introgress disease resistances from this wild species into potato. A series of backcross progenies was developed from a hexaploid somatic hybrid A206. Using a combination of S. brevidens-specific randomly amplified polymorphic DNA (RAPD) markers and a sequential genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) technique, we identified all 12 S. brevidens chromosomes in the backcross progenies. Seven potato-S. brevidens monosomic chromosome addition lines (chromosomes 1, 3, 4, 5, 8, 9 and 10) and one monosomic substitution line (chromosome 6) were identified, and the remaining four S. brevidens chromosomes (2, 7, 11, and 12) were included in two other lines. These chromosomal addition/substitution stocks provide valuable tools for potato cytogenetic research, and can be used to introgress disease resistances from S. brevidens into potato.

摘要

短柄茄是一种野生二倍体马铃薯物种，对几种主要马铃薯病害具有高度抗性。我们之前培育了短柄茄与栽培马铃薯（马铃薯）之间的可育体细胞杂种，以便将该野生物种的抗病性导入马铃薯。从六倍体体细胞杂种A206培育出了一系列回交后代。利用短柄茄特异性随机扩增多态性DNA（RAPD）标记以及连续基因组原位杂交（GISH）和荧光原位杂交（FISH）技术的组合，我们在回交后代中鉴定出了所有12条短柄茄染色体。鉴定出了7个马铃薯-短柄茄单体染色体附加系（染色体1、3、4、5、8、9和10）和1个单体替代系（染色体6），其余4条短柄茄染色体（2、7、11和12）包含在另外两个系中。这些染色体附加/替代材料为马铃薯细胞遗传学研究提供了有价值的工具，可用于将短柄茄的抗病性导入马铃薯。