McGrath J M, Wielgus S M, Helgeson J P
Department of Plant Pathology, University of Wisconsin, Madison 53706, USA.
Genetics. 1996 Apr;142(4):1335-48. doi: 10.1093/genetics/142.4.1335.
The Solanum brevidens genome (2n = 2x = 24) was examined with randomly applied polymorphic DNA (RAPD) markers in a second backcross population derived from a S. brevidens + S. tuberosum somatic hybrid. RAPD markers cosegregated into 12 different S. brevidens synteny groups. Most synteny groups were nonrecombinant. However, nearly 40% of the S. brevidens synteny groups detected in this population were recombinant deletions that carried at least one, but not all, synteny group-specific RAPD markers. All S. brevidens synteny groups (except chromosome 5) were involved in recombination, and recombination occurred within most intervals between markers. About 20% of the recombinant S. brevidens synteny groups involved a single synteny group-specific marker. The inheritance of some single-marker representatives was followed in four BC3 families. At least nine changes in S. brevidens synteny groups had occurred during backcrossing. Six of the nine changes involved translocation of S. brevidens markers between nonhomologous S. brevidens chromosomes, and three S. brevidens markers may have been introgressed into the potato genome.
利用随机应用的多态性DNA（RAPD）标记，对来自马铃薯（Solanum tuberosum）与野生种（Solanum brevidens）体细胞杂种的二次回交群体中的野生种基因组（2n = 2x = 24）进行了检测。RAPD标记共分离成12个不同的野生种同线群。大多数同线群是非重组的。然而，在该群体中检测到的近40%的野生种同线群是重组缺失，这些缺失携带至少一个但不是所有同线群特异性RAPD标记。所有野生种同线群（除了第5号染色体）都参与了重组，并且重组发生在大多数标记之间的区间内。约20%的重组野生种同线群涉及单个同线群特异性标记。在四个BC3家系中追踪了一些单标记代表的遗传情况。在回交过程中，野生种同线群至少发生了九次变化。其中六次变化涉及野生种标记在非同源野生种染色体之间的易位，并且三个野生种标记可能已渗入到马铃薯基因组中。
