Greer L E, Robinson J A, Shelton D R
Pesticide Degradation Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Appl Environ Microbiol. 1992 Mar;58(3):1027-30. doi: 10.1128/aem.58.3.1027-1030.1992.
Seven strains of 2,4-dichlorophenoxyacetic acid-degrading bacteria, including Pseudomonas, Alcaligenes, and Bordetella spp., were compared on the basis of growth kinetics. Estimates of maximum growth rate (mu max, k1) and half-saturation growth constant (Ks, k3) were obtained by fitting substrate depletion curves to a four-parameter version of the integrated Monod equation. Estimates of Ks ranged from 2.2 micrograms/ml (10 microM) to 33.8 micrograms/ml (154 microM), and estimates of mu max ranged from 0.20 h-1 (Td = 3.5 h) to 0.32 h-1 (Td = 2.2 h). Estimates of mu max, but not Ks, were affected by changes in initial inoculum density. Maximum growth rates (mu max) were also estimated from turbidity measurements. They ranged from 0.10 h-1 (Td = 6.9 h) to 1.0 h-1 (Td = 0.7 h). There was no correlation between estimates of mu max derived from substrate depletion curves and those derived from turbidity measurements (P = 0.20).
对包括假单胞菌属、产碱菌属和博德特氏菌属等7株2,4-二氯苯氧乙酸降解菌进行了生长动力学比较。通过将底物消耗曲线拟合到积分莫诺方程的四参数版本，获得了最大生长速率（μm ax，k1）和半饱和生长常数（Ks，k3）的估计值。Ks的估计值范围为2.2微克/毫升（10微摩尔）至33.8微克/毫升（154微摩尔），μm ax的估计值范围为0.20小时-1（Td = 3.5小时）至0.32小时-1（Td = 2.2小时）。μm ax的估计值受初始接种密度变化的影响，而Ks不受影响。最大生长速率（μm ax）也通过浊度测量进行估计。其范围为0.10小时-1（Td = 6.9小时）至1.0小时-1（Td = 0.7小时）。从底物消耗曲线得出的μm ax估计值与从浊度测量得出的估计值之间没有相关性（P = 0.20）。
