Klecka G M, Maier W J
Appl Environ Microbiol. 1985 Jan;49(1):46-53. doi: 10.1128/aem.49.1.46-53.1985.
Batch and fed-batch experiments were conducted to examine the kinetics of pentachlorophenol utilization by an enrichment culture of pentachlorophenol-degrading bacteria. The Haldane modification of the Monod equation was found to describe the relationship between the specific growth rate and substrate concentration. Analysis of the kinetic parameters indicated that the maximum specific growth rate and yield coefficients are low, with values of 0.074 h-1 and 0.136 g/g, respectively. The Monod constant (Ks) was estimated to be 60 micrograms/liter, indicating a high affinity of the microorganisms for the substrate. However, high concentrations (KI = 1,375 micrograms/liter) were shown to be inhibitory for metabolism and growth. These kinetic parameters can be used to define the optimal conditions for the removal of pentachlorophenol in biological treatment systems.
进行了分批和补料分批实验，以研究五氯苯酚降解菌富集培养物对五氯苯酚的利用动力学。发现莫诺德方程的哈代修正能够描述比生长速率与底物浓度之间的关系。动力学参数分析表明，最大比生长速率和产率系数较低，分别为0.074 h-1和0.136 g/g。莫诺德常数（Ks）估计为60微克/升，表明微生物对底物具有高亲和力。然而，高浓度（KI = 1375微克/升）对代谢和生长具有抑制作用。这些动力学参数可用于确定生物处理系统中去除五氯苯酚的最佳条件。
