A straight-forward method of optimising protein solubility for NMR.
作者信息
Howe Peter W A
机构信息
Analytical Sciences, Syngenta, Jealott's Hill Research Centre, Bracknell, Berkshire, RG42 6EY, UK.
出版信息
J Biomol NMR. 2004 Nov;30(3):283-6. doi: 10.1007/s10858-004-3739-4.
PMID:15756459
Abstract
Maximising solubility is a key step in applying solution-state NMR to proteins. The 'microbatch' crystallisation screening method can be adapted to screen for protein solubility. In this approach, drops of test solutions are placed under paraffin oil in 96-well screening plates. This requires very small amounts of protein, is easy to set up and is readily automatable.
摘要
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