次氯酸盐与脂肪酸氢过氧化物的相互作用会导致自由基的产生。

[The interaction of hypochlorite with fatty acid hydroperoxides results in the generation of free radicals].

作者信息

Chekanov A V, Panasenko O M, Osipov A N, Matveeva N S, Kazarinov K D, Vladimirov Iu A, Sergienko V I

出版信息

Biofizika. 2005 Jan-Feb;50(1):13-9.

Abstract

The interaction of hypochlorite with linoleic acid hydroperoxides was studied by the coumarin C-525-enhanced chemiluminescence and ESR spin trapping techniques. Linoleic acid hydroperoxide was obtained in the reaction of lipoxygenase and linoleic acid. Alpha-(4-pyridyl-1-oxyl)-N-tert Butylnitron was used as a spin trap. It was shown that the addition of hypochlorite to the incubation media containing linoleic acid and lipoxygenase resulted in an intensive chemiluminescence flash. The intensity of this flash correlated with the hydroperoxide concentration. The analysis of ESR spectra of spin adducts produced in the reaction of hypochlorite with linoleic acid hydroperoxide showed the presence of O-centered, most likely peroxyl, radical with the splitting constants alphabetaH = 0.260 mT aN = 1.662 mT and C-centered penthyl radical with the splitting constants alphabetaH = 0.260 mT; aN = 1.662 mT. These data suggest that hypochlorite produced by phagocytes in vivo can induce the generation of free O- and C-centered radicals, promoters of free radical processes.

摘要

通过香豆素C - 525增强化学发光和电子自旋共振（ESR）自旋捕获技术研究了次氯酸盐与亚油酸氢过氧化物的相互作用。亚油酸氢过氧化物是在脂氧合酶与亚油酸的反应中获得的。α -（4 - 吡啶基 - 1 - 氧基）-N - 叔丁基硝基被用作自旋捕获剂。结果表明，向含有亚油酸和脂氧合酶的孵育介质中添加次氯酸盐会导致强烈的化学发光闪光。该闪光的强度与氢过氧化物浓度相关。对次氯酸盐与亚油酸氢过氧化物反应中产生的自旋加合物的ESR光谱分析表明，存在以O为中心、最可能是过氧自由基，其分裂常数αβH = 0.260 mT，aN = 1.662 mT，以及以C为中心的戊基自由基，其分裂常数αβH = 0.260 mT；aN = 1.662 mT。这些数据表明，体内吞噬细胞产生的次氯酸盐可诱导产生游离的以O和C为中心的自由基，即自由基过程的促进剂。