Chekanov A V, Panasenko O M, Osipov A N, Arnhold J, Kazarinov K D, Vladimirov Iu A, Sergienko V I
Research Institute of Physical and Chemical Medicine, Malaya Pirogovskaya ul. 1 a, Moscow, 119992 Russia.
Biofizika. 2002 Sep-Oct;47(5):787-94.
The interaction of hypochlorite (HOCl/OCl-) with tert-butyl hydroperoxide ((CH3)3COOH) was investigated by chemiluminescence. It was shown that the addition of HOCl/OCl- to (CH3)3COOH induces a fast chemiluminescent flash. The intensity of this flash increases with the increase in both HOCl/OCl- and (CH3)3COOH concentration. The chemiluminescence is quenched in a concentration-dependent manner in the presence of free radical spin traps N-tert-butyl nitrone and alpha-(4-pyridyl-1-oxyl)-N-tert-butyl nitrone. This fact proves that free radicals take part in the interaction of HOCl/OCl- and (CH3)3COOH. Hypochlorite yielded a very similar chemiluminescence spectrum in its reaction with (CH3)3COOH as Ce4+. It differed considerably from the spectrum in the system H2O2 and HOCl/OCl-. It is well known that the interaction of Ce4+ and (CH3)3COOH produces peroxyl radicals. These results confirm the hyothesis that the interaction of HOCl/OCl- and (CH3)3COOH is mediated by peroxyl radicals. Thus, organic hydroperoxides always present in unsaturated lipids can induce lipid peroxidation processes in the reaction with HOCl/OCl-.
通过化学发光研究了次氯酸盐（HOCl/OCl-）与叔丁基过氧化氢（(CH3)3COOH）的相互作用。结果表明，向(CH3)3COOH中添加HOCl/OCl-会引发快速的化学发光闪光。该闪光的强度随着HOCl/OCl-和(CH3)3COOH浓度的增加而增强。在自由基自旋捕获剂N-叔丁基硝酮和α-(4-吡啶基-1-氧基)-N-叔丁基硝酮存在下，化学发光以浓度依赖的方式猝灭。这一事实证明自由基参与了HOCl/OCl-和(CH3)3COOH的相互作用。次氯酸盐在与(CH3)3COOH反应中产生的化学发光光谱与Ce4+产生的非常相似。它与H2O2和HOCl/OCl-体系中的光谱有很大不同。众所周知，Ce4+与(CH3)3COOH的相互作用会产生过氧自由基。这些结果证实了HOCl/OCl-和(CH3)3COOH的相互作用是由过氧自由基介导的这一假设。因此，不饱和脂质中总是存在的有机氢过氧化物在与HOCl/OCl-反应时会引发脂质过氧化过程。
