Danilova I G, Shevelev I V, Isaev-Ivanov V V, Noskin L A
Biofizika. 2005 Jan-Feb;50(1):49-61.
The effect of catalytic bivalent and inhibitory monovalent cations on the interactions of bovine pancreatic deoxyribonuclease I with the circle single-stranded DNA of M13 phase was studied. It was found that monovalent cations affect the site of binding to DNA and the active center of the enzyme; in their presence, a break of the formation of the enzyme-substrate complex occurs. The results provide evidence that conformational changes of the protein molecule in all cases are more substantial that it could be expected from X-ray data published earlier.
研究了催化性二价阳离子和抑制性一价阳离子对牛胰腺脱氧核糖核酸酶I与M13噬菌体环状单链DNA相互作用的影响。发现一价阳离子会影响酶与DNA的结合位点及酶的活性中心；在它们存在的情况下，酶-底物复合物的形成会受到阻碍。结果表明，在所有情况下蛋白质分子的构象变化都比根据早期发表的X射线数据所预期的更为显著。
