双光子诱导反应中间体的解笼效应。多光子原位检测一种对生物应用具有潜在价值的标记物。

Two-photon induced uncaging of a reactive intermediate. Multiphoton in situ detection of a potentially valuable label for biological applications.

作者信息

Dyer Joanne, Jockusch Steffen, Balsanek Vojtech, Sames Dalibor, Turro Nicholas J

机构信息

Department of Chemistry, Columbia University, New York, New York 10027, USA.

出版信息

J Org Chem. 2005 Mar 18;70(6):2143-7. doi: 10.1021/jo048053c.

DOI:10.1021/jo048053c

PMID:15760198

Abstract

[reaction: see text] Two-photon induced Wolff rearrangement of a terphenyl diazoketone 1 was achieved by using focused laser pulses of 532 nm from a Q-switched Nd:YAG laser. The nonfluorescent terphenyl diazoketone 1 was transformed into a fluorescent ester derivative 4, which can be detected in situ using the focused laser pulses at 532 nm. Laser power dependence studies show that the Wolff rearrangement is induced by two-photon absorption of the terphenyl diazoketone 1, but suggests that more than two photons of 532 nm are involved (a multiphoton process) in excitation of the ester derivative 4.

摘要

[反应：见正文] 通过使用调Q Nd:YAG激光器产生的532 nm聚焦激光脉冲，实现了三联苯重氮酮1的双光子诱导Wolff重排。非荧光三联苯重氮酮1转化为荧光酯衍生物4，该衍生物可使用532 nm聚焦激光脉冲原位检测。激光功率依赖性研究表明，Wolff重排是由三联苯重氮酮1的双光子吸收诱导的，但表明在酯衍生物4的激发过程中涉及超过两个532 nm的光子（多光子过程）。