菁染料作为新型荧光探针用于 DNA 检测和双光子激发生物成像。

Cyanines as new fluorescent probes for DNA detection and two-photon excited bioimaging.

机构信息

Centre for Advanced Luminescence Materials, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.

出版信息

Org Lett. 2010 May 21;12(10):2194-7. doi: 10.1021/ol100474b.

PMID:20405873

Abstract

A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.

摘要

我们合成了一系列基于稠合芳烃的菁类荧光染料作为 DNA 传感和双光子生物成像的电子给体，其中基于咔唑的双菁具有作为荧光点亮探针检测双链 DNA 的高灵敏度和高效率，对富含 AT 的区域表现出选择性结合。双生色骨架的协同效应使双光子吸收截面增强了几个数量级，双链 DNA 结合后双光子激发荧光增强了 25 到 100 倍。