Marletta Giovanni, Ciapetti Gabriela, Satriano Cristina, Pagani Stefania, Baldini Nicola
Laboratory for Molecular Surfaces and Nanotechnology, Dipartimento di Scienze Chimiche, University of Catania, V.le A. Doria 6 - 95125 Catania, Italy.
Biomaterials. 2005 Aug;26(23):4793-804. doi: 10.1016/j.biomaterials.2004.11.047. Epub 2005 Jan 13.
Using techniques of tissue engineering, synthetic substitutes can be applied for the repair and regeneration of damaged bone. It has been found that material surface properties are crucial for cell adhesion and spreading, i.e. cell activities that are related directly to the ability of osteoblasts to proliferate. This fact has promoted the strategy of creating an ECM-like layer onto materials, so as to influence the cell response. In this study human bone-derived osteoblasts have been used to test the effects of surface modification by low energy ion beams of a poly epsilon-caprolactone (PCL) substrate and subsequent RGD adsorption. Osteoblasts were seeded and grown onto untreated and irradiated poly epsilon-caprolactone films, with or without RGD-adsorption step, and viability, morphology, and spreading of the osteoblasts were studied at different time endpoints. Differences were observed in the organization of cytoskeleton within cells: stress fibers were more evident in irradiated samples vs. untreated and total cell adhesion was higher. Surface characterization by X-ray Photoelectron Spectroscopy, Atomic Force Microscopy, and surface free energy measurements showed that the polar character of PCL, i.e., the acid-base term, was increased following irradiation treatment. Moreover the irradiated PCL had a nano-sized topography, which also could improve osteoblasts adhesion. We found that the treatment of the surface with ion beam is per se improving osteoblasts adhesion and spreading onto PCL. Furthermore, also if a significant RGD adsorption was obtained for irradiated PCL surfaces, it was found that in the investigated conditions it seems to have only a minor effect on the cell response. This study suggests that new strategies involving irradiation-based treatments can be adopted to promote the initial steps of bone deposition onto synthetic surfaces, exploiting the surface-induced reorganization of the ECM matrix.
利用组织工程技术，合成替代物可应用于受损骨骼的修复和再生。研究发现，材料表面特性对于细胞黏附和铺展至关重要，即这些细胞活动与成骨细胞的增殖能力直接相关。这一事实推动了在材料上创建类似细胞外基质（ECM）层的策略，以影响细胞反应。在本研究中，人类骨源成骨细胞被用于测试聚ε-己内酯（PCL）基质经低能离子束表面改性及随后RGD吸附的效果。将成骨细胞接种到未处理和经辐照的聚ε-己内酯薄膜上，有或没有RGD吸附步骤，并在不同时间点研究成骨细胞的活力、形态和铺展情况。观察到细胞内细胞骨架组织存在差异：与未处理的样品相比，经辐照的样品中应力纤维更明显，且总细胞黏附更高。通过X射线光电子能谱、原子力显微镜和表面自由能测量进行的表面表征表明，辐照处理后PCL的极性特征，即酸碱项增加。此外，经辐照的PCL具有纳米级形貌，这也可改善成骨细胞的黏附。我们发现，用离子束处理表面本身可改善成骨细胞在PCL上的黏附和铺展。此外，虽然经辐照的PCL表面获得了显著的RGD吸附，但发现在所研究的条件下，它似乎对细胞反应只有轻微影响。本研究表明，可以采用基于辐照处理的新策略，利用表面诱导的ECM基质重组来促进合成表面上骨沉积的初始步骤。
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