Salinas-Carmona M C, Vera L, Welsh O, Rodríguez M
Department of Immunology, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, México.
Zentralbl Bakteriol. 1992 Feb;276(3):390-7. doi: 10.1016/s0934-8840(11)80546-3.
A crude extract from N. brasiliensis cells grown in brain heart infusion culture was analyzed. It showed a complex mixture of at least 37 bands when resolved with the discontinuous buffer system of Laemmli in a gradient SDS-PAGE. Western blot analysis of 16 sera from N. brasiliensis-infected individuals always showed the recognition of six bands of 61, 49, 45, 42, 26, and 24 kilodaltons (kDa). Some other bands also reacted but with less intensity. Sera from tuberculosis and leprosy patients reacted strongly with the 49, 45, and 42 kDa bands but weakly or not at all with the 61, 26, and 24 kDa. Sera from healthy control volunteers reacted with some bands but little or not at all with those three identified by the sera from mycetoma patients. These three immunodominant antigens (61, 26 and 24 kDa) may be of clinical value in the serodiagnosis of mycetoma by N. brasiliensis.
对在脑心浸液培养基中生长的巴西奴卡菌细胞的粗提物进行了分析。当在梯度SDS-PAGE中用Laemmli不连续缓冲系统进行分离时，它显示出至少37条带的复杂混合物。对来自巴西奴卡菌感染个体的16份血清进行的蛋白质印迹分析始终显示出对6条分子量分别为61、49、45、42、26和24千道尔顿（kDa）条带的识别。其他一些条带也有反应，但强度较低。肺结核和麻风病患者的血清与49、45和42 kDa条带强烈反应，但与61、26和24 kDa条带反应较弱或根本不反应。健康对照志愿者的血清与一些条带反应，但与足菌肿患者血清识别出的那三条条带反应很少或根本不反应。这三种免疫显性抗原（61、26和24 kDa）可能在巴西奴卡菌引起的足菌肿的血清诊断中具有临床价值。
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