Castro-Matteotti Bárbara, Vera-Cabrera Lucio, Ocampo-Candiani Jorge, Rendón Adrián, Salinas-Carmona Mario C, Welsh Oliverio
Servicio de Dermatología, Hospital Universitario José E. González, UANL, Madero y Gonzalitos, Col. Mitras Centro, Monterrey, N.L, CP 64710, México.
Mycopathologia. 2008 Mar;165(3):127-34. doi: 10.1007/s11046-008-9093-4. Epub 2008 Feb 27.
The ability of culture-filtrate proteins to induce a cellular immune response in infected mice and humans was investigated. A crude extract culture filtrate of Nocardia brasiliensis (CFA) and five semi-purified CFA fractions (P1, P2, P3, P4, P5) were used to stimulate BALB/c mice spleen-cell cultures. The animals were divided into three groups: the first group was infected with 1 x 10(7) CFU of N. brasiliensis in the footpad, the second group was immunized with heat-killed bacteria, and the third was injected with sterile saline. IFN-gamma, IL-1alpha, and IL-4 concentrations were determined in culture supernatants. Protein fractions eliciting IFN-gamma production in mice, as well as the CFA, were used to stimulate IFN-gamma production and in vitro cell proliferation assays with peripheral blood mononuclear cells of patients with actinomycetoma by N. brasiliensis, individuals with pulmonary tuberculosis, and healthy controls. In mice, CFA and three of the protein fractions (P3, P4 and P5) induced significant IFN-gamma production in the infected group. In humans, only the CFA-induced IFN-gamma production and cell proliferation in the group of patients with actinomycetoma. There was no stimulation in tuberculosis patients nor healthy controls. These results suggest that some culture-filtrate antigens are recognized by patients with active actinomycetoma and do not cross-react with M. tuberculosis antigens, being therefore potential candidates to develop a diagnostic test.
研究了巴西诺卡菌培养滤液蛋白在感染小鼠和人类中诱导细胞免疫反应的能力。使用巴西诺卡菌的粗提物培养滤液（CFA）和五个半纯化的CFA组分（P1、P2、P3、P4、P5）刺激BALB/c小鼠脾细胞培养物。动物被分为三组：第一组在足垫接种1×10⁷CFU的巴西诺卡菌，第二组用热灭活细菌免疫，第三组注射无菌盐水。测定培养上清液中的IFN-γ、IL-1α和IL-4浓度。用在小鼠中诱导IFN-γ产生的蛋白组分以及CFA，对巴西诺卡菌所致放线菌瘤患者、肺结核患者和健康对照者的外周血单个核细胞进行IFN-γ产生刺激和体外细胞增殖试验。在小鼠中，CFA和三个蛋白组分（P3、P4和P5）在感染组中诱导显著的IFN-γ产生。在人类中，只有CFA在放线菌瘤患者组中诱导IFN-γ产生和细胞增殖。在肺结核患者和健康对照者中未观察到刺激作用。这些结果表明，一些培养滤液抗原可被活动性放线菌瘤患者识别，且不与结核分枝杆菌抗原发生交叉反应,因此是开发诊断试验的潜在候选物。
