Kasai K, Ishii T, Sato S
Department of Biology and Earth Sciences, Faculty of Science, Ehime University, Matsuyama, Japan.
Biotech Histochem. 2004 Oct-Dec;79(5-6):163-7. doi: 10.1080/10520290400016736.
We designed two procedures to visualize simultaneously clusters of ribosomal RNA genes (rDNA) and the nucleolus in plant cells. The procedures combine fluorescence in situ hybridization (FISH) to visualize the rDNA clusters and silver staining to observe the nucleolus. When FISH is followed by silver staining, many minute FISH signals are localized in the nucleolus, and several large FISH signals are seen on the nucleolar periphery. When FISH was applied to the specimens with silver nitrate staining, large FISH signals were visualized in the nucleoplasm associated with the nucleolar periphery, but no signals were seen in the nucleoli. Thus, the two combinations of FISH and silver staining provided different details regarding the arrangement of rDNA clusters in the nucleolus of plant cells.
我们设计了两种方法，用于同时观察植物细胞中核糖体RNA基因（rDNA）簇和核仁。这些方法结合了荧光原位杂交（FISH）来观察rDNA簇，以及银染来观察核仁。当先进行FISH再进行银染时，许多微小的FISH信号位于核仁中，并且在核仁周边可见几个大的FISH信号。当将FISH应用于经硝酸银染色的标本时，大的FISH信号在与核仁周边相关的核质中可见，但在核仁中未见信号。因此，FISH和银染的两种组合提供了关于植物细胞核仁中rDNA簇排列的不同细节。
