Pazur J H, Li N-Q
Department of Biochemistry and Molecular Biology, 108 Althouse Laboratory, Pennsylvania State University, University Park, PA 16802, USA.
Food Addit Contam. 2004 Nov;21(11):1027-34. doi: 10.1080/02652030400019406.
Anti-carbohydrate antibodies with specificities for polysaccharide gums were isolated from the serum of rabbits that were immunized with a solution of the gums and Freund's complete adjuvant. The primary objective was to test an immunological method for the detection of the polysaccharide gums as additives to processed foods. Analysis involved the extraction of food with phosphate buffer and the testing of the extract for a reaction with anti-gum antibodies by the agar diffusion method. Reaction by a specific gum with the homologous antibodies establishes the presence of the gum in the food. The method is a novel application of antibodies. The antibody method is highly specific for a gum and thus possesses advantages over other methods of analysis for polysaccharide gums as additives in processed foods.
从用多糖胶溶液和弗氏完全佐剂免疫的兔子血清中分离出对多糖胶具有特异性的抗碳水化合物抗体。主要目的是测试一种用于检测作为加工食品添加剂的多糖胶的免疫学方法。分析包括用磷酸盐缓冲液提取食品，并用琼脂扩散法检测提取物与抗胶抗体的反应。特定的胶与同源抗体发生反应表明食品中存在该胶。该方法是抗体的一种新应用。抗体方法对胶具有高度特异性，因此相对于其他分析加工食品中作为添加剂的多糖胶的方法具有优势。
