Yang H Y, Lieska N, Goldman R D, Johnson-Seaton D, Pappas G D
Department of Anatomy and Cell Biology, College of Medicine, University of Illinois, Chicago 60612.
Brain Res. 1992 Feb 21;573(1):161-8. doi: 10.1016/0006-8993(92)90127-u.
The nature and tissue origin of cultured non-stellate astrocytes have not been defined. On the basis of immunofluorescence microscopy using multiple double-labeling with antibodies to glial fibrillary acidic protein (GFAP), vimentin, and the recently identified intermediate filament-associated protein (IFAP)-70/280kD, four distinct astrocytic subtypes were definable in neonatal rat brain astrocytes in culture. All of these were of the non-stellate type on the basis of morphology. Similar examination of developing rat cerebral cortex identified these same subtypes as distinct differentiation states of astrocytes. These findings indicate that the parallel developmental events can be studied in vitro.
培养的非星状星形胶质细胞的性质和组织来源尚未明确。基于使用针对胶质纤维酸性蛋白（GFAP）、波形蛋白以及最近鉴定出的中间丝相关蛋白（IFAP）-70/280kD的抗体进行多重双标记的免疫荧光显微镜检查，在培养的新生大鼠脑星形胶质细胞中可确定四种不同的星形胶质细胞亚型。基于形态学，所有这些亚型均为非星状类型。对发育中的大鼠大脑皮层进行类似检查，确定这些相同的亚型为星形胶质细胞的不同分化状态。这些发现表明，可以在体外研究平行的发育事件。
