Loh K C, Wang S J
Department of Chemical Engineering National University of Singapore, 10 Kent Ridge Crescent, S119260, Singapore.
Biodegradation. 1997;8(5):329-38. doi: 10.1023/a:1008267607634.
The enhancement of biodegradation of phenol and 4-chlorophenol (4-cp) as a cometabolised compound by Pseudomonas putida ATCC 49451 was accomplished by augmenting the medium with conventional carbon sources such as sodium glutamate and glucose. Compared with phenol as the sole carbon source, the addition of 1 gl(-1) sodium glutamate increased the toxicity tolerance of cells toward 4-cp and significantly improved the biodegradation rates of both phenol and 4-cp even when the initial concentration of 4-cp was as high as 200 mgl(-1). On the other hand, supplementation of glucose caused a significant drop in the medium pH from 7.2 to 4.3 resulting in a reduction of degradation rate, leaving a considerable amount of 4-cp undegraded when the initial concentration of 4-cp was higher than 100 mgl(-1). By regulating the pH of the medium, however, enhancement of degradation rates of phenol and 4-cp in the presence of glucose was achieved with a concomitant complete degradation of phenol and 4-cp.
恶臭假单胞菌ATCC 49451作为共代谢化合物对苯酚和4-氯苯酚（4-CP）生物降解的增强作用，是通过向培养基中添加传统碳源如谷氨酸钠和葡萄糖来实现的。与以苯酚作为唯一碳源相比，添加1 g l⁻¹谷氨酸钠提高了细胞对4-CP的毒性耐受性，并且即使在4-CP初始浓度高达200 mg l⁻¹时，也显著提高了苯酚和4-CP的生物降解率。另一方面，添加葡萄糖导致培养基pH值从7.2显著降至4.3，从而导致降解率降低，当4-CP初始浓度高于100 mg l⁻¹时，会留下大量未降解的4-CP。然而，通过调节培养基的pH值，在葡萄糖存在的情况下实现了苯酚和4-CP降解率的提高，同时苯酚和4-CP也完全降解。
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