School of Pharmacy, Queen's University of Belfast, Belfast, United Kingdom.
PLoS One. 2013;8(4):e60806. doi: 10.1371/journal.pone.0060806. Epub 2013 Apr 1.
The aim of this study was to isolate and identify marine-derived bacteria which exhibited high tolerance to, and an ability to biodegrade, 1-alkyl-3-methylimidazolium chloride ionic liquids. The salinity and hydrocarbon load of some marine environments may induce selective pressures which enhance the ability of microbes to grow in the presence of these liquid salts. The isolates obtained in this study generally showed a greater ability to grow in the presence of the selected ionic liquids compared to microorganisms described previously, with two marine-derived bacteria, Rhodococcus erythropolis and Brevibacterium sanguinis growing in concentrations exceeding 1 M 1-ethyl-3-methylimidazolium chloride. The ability of these bacteria to degrade the selected ionic liquids was assessed using High Performance Liquid Chromatography (HPLC), and three were shown to degrade the selected ionic liquids by up to 59% over a 63-day test period. These bacterial isolates represent excellent candidates for further potential applications in the bioremediation of ionic liquid-containing waste or following accidental environmental exposure.
本研究旨在分离和鉴定对 1-烷基-3-甲基咪唑氯离子液体具有高耐受性和生物降解能力的海洋来源细菌。一些海洋环境的盐度和碳氢化合物负荷可能会产生选择压力，增强微生物在这些液体盐存在下生长的能力。本研究中获得的分离物通常显示出比以前描述的微生物在存在所选离子液体时更大的生长能力，两种海洋来源的细菌，红球菌（Rhodococcus erythropolis）和短杆菌（Brevibacterium sanguinis）在浓度超过 1 M 的 1-乙基-3-甲基咪唑氯中生长。使用高效液相色谱法（HPLC）评估了这些细菌降解所选离子液体的能力，结果表明其中三种细菌在 63 天的测试期内可将所选离子液体降解多达 59%。这些细菌分离物是进一步在离子液体废物的生物修复或意外环境暴露后的潜在应用的优秀候选物。
