Restani Patrizia, Ballabio Cinzia, Corsini Emanuela, Fiocchi Alessandro, Isoardi Patrizia, Magni Chiara, Poiesi Claudio, Terracciano Luigi, Duranti Marcello
Department of Pharmacological Sciences, University of Milano, Milano, Italy.
Ann Allergy Asthma Immunol. 2005 Feb;94(2):262-6. doi: 10.1016/S1081-1206(10)61306-3.
Several proteins have been identified as peanut allergens; among them, Ara h 1 (7S globulin) and Ara h 2 (2S globulin) are usually considered the major allergens.
To identify the major allergens in a group of children selected for their specific pattern of immunoreactivity.
We identified the dominant allergen by using (1) amino acid sequencing of the bands that show the strongest IgE immunoreactivity in 1-dimensional electrophoresis and immunoblotting and (2) specific animal IgGs raised against the dominant immunoreactive band to pinpoint the allergen(s) in peanut proteins separated by 2-dimensional electrophoresis and immunoblotting. To confirm these data, we further examined the peanut proteome using serum samples from the children with the unusual immunoreactivity.
We found a group of children with marked peanut allergy who are specifically sensitized to the basic subunit of Ara h 3 (11S globulin family).
That the dominant immunoreactivity in these patients is in a basic subunit of Ara h 3 was unexpected, because previous studies had indicated that Ara h 3 was only a minor peanut allergen and that the identified allergenic epitopes occurred mainly in the acidic Ara h 3 subunit.
几种蛋白质已被鉴定为花生过敏原；其中，Ara h 1（7S球蛋白）和Ara h 2（2S球蛋白）通常被认为是主要过敏原。
在一组因其特定免疫反应模式而被挑选出的儿童中鉴定主要过敏原。
我们通过以下方法鉴定主要过敏原：（1）对在一维电泳和免疫印迹中显示最强IgE免疫反应性的条带进行氨基酸测序；（2）使用针对主要免疫反应性条带产生的特异性动物IgG，以确定二维电泳和免疫印迹分离的花生蛋白中的过敏原。为了证实这些数据，我们使用具有异常免疫反应性的儿童的血清样本进一步检测花生蛋白质组。
我们发现一组对花生有明显过敏反应的儿童，他们对Ara h 3（11S球蛋白家族）的碱性亚基有特异性致敏。
这些患者中的主要免疫反应性位于Ara h 3的碱性亚基，这是出乎意料的，因为先前的研究表明Ara h 3只是一种次要的花生过敏原，且已鉴定的过敏原表位主要出现在酸性的Ara h 3亚基中。
