Horiguchi Shigetoshi, Okamoto Yoshitaka, Chazono Hideaki, Sakurai Daiju, Kobayashi Kouichi
Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Ann Allergy Asthma Immunol. 2005 Feb;94(2):286-91. doi: 10.1016/S1081-1206(10)61310-5.
CD23 is the low-affinity receptor for IgE on B cells and is thought to play an important role in regulation of IgE production.
To measure the expression of membrane-bound CD23 in nasal B cells and examine its correlation with CD4 subtypes or serum IgE levels in patients with perennial allergic rhinitis.
We used flow cytometric analysis with double, direct immunofluorescence staining of the mucosal-infiltrating lymphocytes to examine the expression of CD23 in nasal mucosal B cells of patients with perennial allergic rhinitis. The expression of CD23 in nasal B cells of patients with nonatopic rhinosinusitis served as a control.
The ratio of CD23+ B cells to total B cells in patients with perennial allergic rhinitis was significantly higher than in nonatopic controls, whereas that of B cells to total lymphocytes was unchanged. The ratio of CCR4+ CD4 cells to total CD4 cells in allergic patients was significantly higher than in nonatopic controls, whereas the ratio of CXCR3+ CD4 cells to total CD4 cells was unchanged. There was no significant correlation between the percentages of CD23+ B cells and CCR4+ CD4 cells. In addition, the percentage of CD23+ B cells did not correlate with the total IgE level or with the specific IgE level.
Our results indicate that nasal mucosal CD23-bearing B cells, as well as T(H)2 cells, increase in patients with perennial allergic rhinitis. However, the expression of CD23 did not directly correlate with the number of T(H)2 cells in the nasal mucosa.
CD23是B细胞上IgE的低亲和力受体，被认为在IgE产生的调节中起重要作用。
检测常年性变应性鼻炎患者鼻B细胞中膜结合型CD23的表达，并探讨其与CD4亚型或血清IgE水平的相关性。
我们采用流式细胞术分析和对黏膜浸润淋巴细胞进行双重直接免疫荧光染色，以检测常年性变应性鼻炎患者鼻黏膜B细胞中CD23的表达。将非变应性鼻窦炎患者鼻B细胞中CD23的表达作为对照。
常年性变应性鼻炎患者中CD23+B细胞与总B细胞的比例显著高于非变应性对照组，而B细胞与总淋巴细胞的比例未变。变应性患者中CCR4+CD4细胞与总CD4细胞的比例显著高于非变应性对照组，而CXCR3+CD4细胞与总CD4细胞的比例未变。CD23+B细胞百分比与CCR4+CD4细胞百分比之间无显著相关性。此外，CD23+B细胞百分比与总IgE水平或特异性IgE水平均无相关性。
我们的结果表明，常年性变应性鼻炎患者鼻黏膜中携带CD23的B细胞以及辅助性T细胞2（Th2）细胞增多。然而，CD23的表达与鼻黏膜中Th2细胞数量无直接相关性。
