Vashchenko L N, Litvinchuk O A, Gvozdiak R I, Votselko S K
Mikrobiol Z. 2004 Nov-Dec;66(6):37-43.
A possibility to use a new polymer--exopolysaccharide acrylamide (EPAA)--for partial substitution of agar-agar in solid nutrient media in microbiology has been studied. It has been established than the EPAA in concentration 6.0-12.0 g/l with agar in concentration 10.0 g/l produce gel which is so strong as agar in concentration 15.0-20.0 g/l. Quality of gels produced depends an the method of EPAA mixing with agar. It has been shown that usage of EPAA in nutrient media does not affect growth, colony and cell morphology and biochemical properties of microorganisms. The use of EPAA to prepare nutrient media permits us to reduce expenditures for agar.
研究了使用一种新型聚合物——胞外多糖丙烯酰胺（EPAA）——部分替代微生物学固体营养培养基中琼脂的可能性。已确定浓度为6.0 - 12.0 g/l的EPAA与浓度为10.0 g/l的琼脂可制成凝胶，其强度与浓度为15.0 - 20.0 g/l的琼脂制成的凝胶相当。所制凝胶的质量取决于EPAA与琼脂的混合方法。结果表明，在营养培养基中使用EPAA不会影响微生物的生长、菌落和细胞形态以及生化特性。使用EPAA制备营养培养基可以降低琼脂的成本。
