Sun Hang, Wu Chuan-xin, Gong Jian-ping, Liu Hai-zhong, Li Xu-hong, You Hai-bo, Chen Xian-feng
Institute of Viral Hepatitis, 2nd Affiliate Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Mar;21(2):155-8.
To observe the 1, 25-dihydroxy vitamin D3 (Vit D3) induced CD14 expression in human U937 cell line and the reaction of the cells to LPS stimulation following the induction.
U937 cells were cocultured with 0.1 mumol/L Vit D3 for 24 hours to induce the expression of CD14 gene and the sensitiveness of U937 cells to stimulation of LPS at various concentrations and for different times were observed.
Vit D3 stably induced U937 cells to express CD14 mRNA and CD14 protein. The sensitivity of U937 cells to LPS stimulation increased notably after Vit D3 induction. It was demonstrated that low concentration of LPS stimulated the activation of NF-kappaB in U937/CD14 cells, and enhanced the transcription and expression of TNF-alpha gene, and even release of TNF-alpha into the culture supernatant.
Vit D3 can induce U937 cells to express CD14 gene and CD14 protein and enhance the reactivity of U937/CD14 cells to LPS stimulation.
观察1,25 - 二羟基维生素D3（Vit D3）诱导人U937细胞系中CD14表达以及诱导后细胞对脂多糖（LPS）刺激的反应。
将U937细胞与0.1 μmol/L Vit D3共培养24小时以诱导CD14基因表达，观察不同浓度LPS在不同时间刺激下U937细胞的敏感性。
Vit D3稳定诱导U937细胞表达CD14 mRNA和CD14蛋白。Vit D3诱导后U937细胞对LPS刺激的敏感性显著增加。结果表明，低浓度LPS刺激U937/CD14细胞中NF-κB的激活，增强TNF-α基因的转录和表达，甚至使TNF-α释放到培养上清液中。
Vit D3可诱导U937细胞表达CD14基因和CD14蛋白，并增强U937/CD14细胞对LPS刺激的反应性。
