Kushnir Mark M, Rockwood Alan L, Nelson Gordon J, Yue Bingfang, Urry Francis M
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA.
Clin Biochem. 2005 Apr;38(4):319-27. doi: 10.1016/j.clinbiochem.2004.12.003.
The necessity of confirmation of compound identity in quantitative analysis is well recognized for methods utilizing single mass spectrometry detection but is not commonly addressed for applications utilizing multiple-stage mass spectrometry (MSn). For MSn detection, no commonly accepted rules for assessment of analytical specificity in quantitative analyses have been established to date.
To assure compound identity, we evaluated approaches based on monitoring multiple mass transitions of a target compound followed by comparison of the branching ratios of the mass transitions.
Monitoring multiple mass transitions along with evaluation of the ratio of their relative intensities allows the analyst to distinguish the target analyte from interferences in quantitative analysis. The strategy and the acceptance criteria are compound and method specific and should be established during the method development and validation.
The certainty of analyte identity is very important in quantitative analysis using MSn detection; methods to verify analyte identity should be used in all critical applications.
利用单重质谱检测的方法在定量分析中确认化合物身份的必要性已得到充分认可，但在利用多级质谱（MSn）的应用中通常未得到解决。对于MSn检测，迄今为止尚未建立定量分析中评估分析特异性的普遍接受的规则。
为确保化合物身份，我们评估了基于监测目标化合物的多个质量跃迁并随后比较质量跃迁分支比的方法。
监测多个质量跃迁并评估其相对强度的比率使分析人员能够在定量分析中区分目标分析物和干扰物。该策略和验收标准因化合物和方法而异，应在方法开发和验证期间确定。
在使用MSn检测的定量分析中，分析物身份的确定性非常重要；应在所有关键应用中使用验证分析物身份的方法。
