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通过血沉棕黄层聚合酶链反应分析诊断的犬全身性鸟分枝杆菌感染

Systemic Mycobacterium avium infection in a dog diagnosed by polymerase chain reaction analysis of buffy coat.

作者信息

Naughton James F, Mealey Katrina L, Wardrop K Jane, Oaks J Lindsay, Bradway Daniel S

机构信息

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-6610, USA>

出版信息

J Am Anim Hosp Assoc. 2005 Mar-Apr;41(2):128-32. doi: 10.5326/0410128.

DOI:10.5326/0410128
PMID:15767657
Abstract

Dogs may be infected by Mycobacterium (M.) tuberculosis, M. bovis, and M. avium complex, and the clinical signs associated with each of these infections may be indistinguishable. Rapid speciation of the infecting organism is desirable because of the public health concerns associated with M. bovis and M. tuberculosis infections. A mycobacterial infection was suspected in the dog of this report based on acid-fast staining of organisms in macrophages obtained from liver aspirates and buffy-coat preparations. Polymerase chain reaction (PCR) analysis of a buffy-coat preparation identified M. avium.

摘要

犬类可能感染结核分枝杆菌、牛分枝杆菌和鸟分枝杆菌复合群,且与这些感染相关的临床症状可能难以区分。鉴于牛分枝杆菌和结核分枝杆菌感染所涉及的公共卫生问题,对感染病原体进行快速鉴定很有必要。根据从肝脏穿刺物和血沉棕黄层制备物中获得的巨噬细胞内病原体的抗酸染色结果,怀疑本报告中的犬患有分枝杆菌感染。对血沉棕黄层制备物进行聚合酶链反应(PCR)分析,鉴定出为鸟分枝杆菌。

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