从新西兰绵羊流产病例中分离出的胎儿弯曲杆菌胎儿亚种的脉冲场凝胶电泳分型
Pulsed-field gel electrophoresis typing of Campylobacter fetus subsp. fetus isolated from sheep abortions in New Zealand.
作者信息
Mannering S A, West D M, Fenwick S G, Marchant R M, Perkins N R, O'connell K
机构信息
Institute of Veterinary Animal and Biomedical Sciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.
出版信息
N Z Vet J. 2004 Dec;52(6):358-63. doi: 10.1080/00480169.2004.36452.
AIMS
To genotype Campylobacter fetus subsp. fetus isolates cultured from sheep abortions submitted to diagnostic laboratories in New Zealand during the year 2000 breeding season. To compare the types found nationally with those found in the Hawke's Bay region in 1999, and strains held in the New Zealand Reference Culture Collection, Medical Section (NZRM) from a study published in 1987.
METHODS
Campylobacter fetus subsp. fetus isolates cultured by veterinary diagnostic laboratories in the year 2000 breeding season, from sheep abortions from throughout New Zealand, were typed using pulsed-field gel electrophoresis (PFGE). In addition, seven freeze-dried C. fetus subsp. fetus isolates (strain numbers 2939-2945) from the NZRM, representing restriction types a-g found amongst sheep abortion isolates in a study published in 1987, were typed using PFGE.
RESULTS
In total, 293 C. fetus subsp. fetus isolates from 200 farms were obtained from veterinary diagnostic laboratories. Twenty-two distinct PFGE profiles were identified amongst the isolates. PFGE type B1 was predominant in each region of New Zealand and was identified from 66% of farms overall. Of the C. fetus subsp. fetus restriction types a-g lodged with the NZRM, 3/7 had PFGE profiles indistinguishable from profiles found in the current study. The other four restriction types had PFGE profiles that were unique but similar to those found in the current study.
CONCLUSIONS
PFGE type B1 was predominant amongst the C. fetus subsp. fetus isolates cultured from sheep abortions in each region of New Zealand in the year 2000, as was found in Hawke's Bay in 1999. The similarity between PFGE profiles of C. fetus subsp. fetus sheep abortion isolates from 1987 and 2000, and the relative prevalence of the PFGE groups, suggests that there has been no major genotypic shift in the population of C. fetus subsp. fetus implicated in sheep abortion in New Zealand during this time.
目的
对2000年繁殖季节提交给新西兰诊断实验室的、从绵羊流产病例中培养出的胎儿弯曲菌胎儿亚种分离株进行基因分型。将全国范围内发现的菌株类型与1999年在霍克斯湾地区发现的类型以及1987年发表的一项研究中保存在新西兰医学参考培养物保藏中心（NZRM）的菌株进行比较。
方法
对2000年繁殖季节由兽医诊断实验室从新西兰各地绵羊流产病例中培养出的胎儿弯曲菌胎儿亚种分离株，采用脉冲场凝胶电泳（PFGE）进行分型。此外，对来自NZRM的7株冻干胎儿弯曲菌胎儿亚种分离株（菌株编号2939 - 2945）进行PFGE分型，这些分离株代表了1987年发表的一项研究中在绵羊流产分离株中发现的限制性类型a - g。
结果
共从兽医诊断实验室获得了来自200个农场的293株胎儿弯曲菌胎儿亚种分离株。在这些分离株中鉴定出22种不同的PFGE图谱。PFGE B1型在新西兰的每个地区都占主导地位，总体上从66%的农场中鉴定出该类型。在保藏于NZRM的胎儿弯曲菌胎儿亚种限制性类型a - g中，7株中有3株的PFGE图谱与本研究中发现的图谱无法区分。其他四种限制性类型具有独特但与本研究中发现的图谱相似的PFGE图谱。
结论
2000年，PFGE B1型在新西兰各地区从绵羊流产病例中培养出的胎儿弯曲菌胎儿亚种分离株中占主导地位，1999年在霍克斯湾地区也发现了这种情况。1987年和2000年胎儿弯曲菌胎儿亚种绵羊流产分离株的PFGE图谱相似，以及PFGE组的相对流行情况表明，在此期间，新西兰涉及绵羊流产的胎儿弯曲菌胎儿亚种群体中没有发生重大的基因型转变。
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