Meng X J, Qiu B A, Li X J, Song X H
Institute of Basic Medical Sciences, General Hospital of PLA, Beijing.
Chin Med J (Engl). 1992 Jan;105(1):34-8.
The effect of Kupffer cell blockade on hepatic function during sepsis was evaluated in this study. Methyl palmitate suspension 100 mg/100 g administered intravenously suppressed the phagocytic activity as the phagocytic index K decreased from 0.0493 +/- 0.0089 to 0.0150 +/- 0.0035 in rats. Sepsis was produced by the method of cecal ligation and needling perforation (CLP). At the end of 15 hours after CLP the hepatic adenosine triphosphate (ATP) level and ketone body ratio decreased significantly. But in rats pretreated with methyl palmitate 24 hours prior to CLP, the ATP level returned to the normal control level (1.6906 +/- 0.06-2.2323 +/- 0.13 mumol/g) and ketone body ratio remained at significantly higher values (0.26 to 0.68). After CLP, the liver lipoperoxide (LPO) concentration increased and glutathione (GSH) contents decreased significantly. When the septic rats were pretreated with methyl palmitate, both the LPO and GSH returned to the normal control level (62.69 +/- 1.7 to 44.62 +/- 2.12 and 159.85 +/- 9.7 to 222.27 +/- 11.34). It is concluded that the hepatic dysfunction is modulated at least to a greater extent by many of the toxic mediators released by the activated Kupffer cells during sepsis.
本研究评估了库普弗细胞阻断对脓毒症期间肝功能的影响。静脉注射100mg/100g的棕榈酸甲酯悬浮液可抑制吞噬活性，大鼠的吞噬指数K从0.0493±0.0089降至0.0150±0.0035。采用盲肠结扎穿刺（CLP）法制造脓毒症模型。CLP术后15小时末，肝脏三磷酸腺苷（ATP）水平和酮体比率显著降低。但在CLP前24小时用棕榈酸甲酯预处理的大鼠中，ATP水平恢复到正常对照水平（1.6906±0.06 - 2.2323±0.13μmol/g），酮体比率仍显著高于正常水平（0.26至0.68）。CLP后，肝脏脂质过氧化物（LPO）浓度升高，谷胱甘肽（GSH）含量显著降低。当脓毒症大鼠用棕榈酸甲酯预处理后，LPO和GSH均恢复到正常对照水平（62.69±1.7至44.62±2.12以及159.85±9.7至222.27±11.34）。得出的结论是，脓毒症期间活化的库普弗细胞释放的许多毒性介质至少在很大程度上调节了肝功能障碍。
