Ke Wei-zhong, Wu Jian-zhong
Nanjing Normal University, Jiangsu Provincial Key Lab for Optoelectronic Technology, Nanjing 210097, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2004 May;24(5):551-3.
The surface-enhanced Raman spectra of glycine, valine and lysine adsorbed on silver colloidal particles and the Raman spectrum of lysine in aqueous solution were obtained. The adsorption was accompanied by the appearance of the 238 cm(-1) band corresponding to the oscillation of the molecule-metal chemical bond and indicating chemisorption of the amino acid. In the surface-enhanced Raman spectra of three amino acids, the peak bands at 900-930 cm(-1), near 1100 cm(-1) and near 1400 cm(-1), corresponding to the carbon-carbon bond, carboxyl and amino group vibrations, respectively, were markedly increased on the silver colloidal particles. It means that the Raman spectra of polar groups such as COO-, N2 and C-COO- were strongly enhanced via the carboxyl group. There was no influence under the low pH condition in the experimental system used.
获得了吸附在银胶体颗粒上的甘氨酸、缬氨酸和赖氨酸的表面增强拉曼光谱以及赖氨酸在水溶液中的拉曼光谱。吸附过程伴随着对应于分子 - 金属化学键振动的238 cm(-1)谱带的出现，这表明氨基酸发生了化学吸附。在三种氨基酸的表面增强拉曼光谱中，分别对应于碳 - 碳键、羧基和氨基振动的900 - 930 cm(-1)、1100 cm(-1)附近和1400 cm(-1)附近的峰带在银胶体颗粒上明显增强。这意味着诸如COO-、N2和C - COO-等极性基团的拉曼光谱通过羧基得到了强烈增强。在所使用的实验系统中，低pH条件下没有影响。
