Capaccio Marcello, Gavalas Vasilis G, Meier Mark S, Anthony John E, Bachas Leonidas G
Department of Chemistry and Advanced Carbon Materials Center, University of Kentucky, Lexington, Kentucky 40506-0055, USA.
Bioconjug Chem. 2005 Mar-Apr;16(2):241-4. doi: 10.1021/bc049861d.
A novel biotinylated fullerene has been synthesized to facilitate the attachment of biotin-conjugated proteins to C(60) through the use of streptavidin as a molecular adapter. The strong biotin-streptavidin interaction enables the attachment of fullerenes to streptavidin and, because of the availability of four biotin-binding sites on streptavidin, to biotinylated biomolecules. The feasibility of this approach is demonstrated by using biotinylated alkaline phosphatase. Due to the insolubility of fullerenes in aqueous media, the immobilized enzyme can be eventually recovered by simple centrifugation with no significant loss in activity.
一种新型生物素化富勒烯已被合成，通过使用链霉亲和素作为分子适配器，促进生物素共轭蛋白与C(60)的连接。生物素与链霉亲和素之间的强相互作用使得富勒烯能够与链霉亲和素连接，并且由于链霉亲和素上有四个生物素结合位点，富勒烯也能够与生物素化生物分子连接。通过使用生物素化碱性磷酸酶证明了该方法的可行性。由于富勒烯在水性介质中不溶，固定化酶最终可通过简单离心回收，且活性无显著损失。
