Fukase N, Takahashi H, Manaka H, Igarashi M, Yamatani K, Daimon M, Sugiyama K, Tominaga M, Sasaki H
Third Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Diabetes Res Clin Pract. 1992 Mar;15(3):187-95. doi: 10.1016/0168-8227(92)90024-l.
To investigate the mechanism of oral carbohydrate-stimulated secretion of the two most potent incretin candidates, gastric inhibitory polypeptide (GIP) and truncated glucagon-like peptide-1 (tGLP-1), we studied the changes in the plasma levels of these peptides in five healthy men after sucrose ingestion with or without pretreatment with an alpha-D-glucosidase inhibitor (AO-128). After sucrose ingestion, plasma levels of GIP peaked at 15 min and remained high up to 120 min. Plasma levels of GLP-1 NT measured with antiserum R1043 (N-terminal specific) tended to decrease gradually and those of GLP-1 CT measured with antiserum R2337 (C-terminal specific) increased. Therefore, estimated plasma levels of tGLP-1 increased markedly within 30 min, then declined slightly over the next 60 min. After treatment with AO-128 (0.6 mg/day) for 1 week, increases in plasma glucose and insulin levels were attenuated and the increase in plasma GIP levels was diminished, while the increase in tGLP-1 levels was sustained much longer. It is concluded that GIP secretion is stimulated by glucose absorption and tGLP-1 secretion by the presence of sucrose in the gut.
为了研究口服碳水化合物刺激两种最有效的肠促胰岛素候选物——胃抑制性多肽（GIP）和截短的胰高血糖素样肽-1（tGLP-1）分泌的机制，我们研究了5名健康男性在摄入蔗糖时或预先使用α-D-葡萄糖苷酶抑制剂（AO-128）处理后这些肽的血浆水平变化。摄入蔗糖后，GIP的血浆水平在15分钟时达到峰值，并在120分钟内一直保持在较高水平。用抗血清R1043（N端特异性）测定的GLP-1 NT血浆水平倾向于逐渐下降，而用抗血清R2337（C端特异性）测定的GLP-1 CT血浆水平则升高。因此，估计的tGLP-1血浆水平在30分钟内显著升高，然后在接下来的60分钟内略有下降。用AO-128（0.6毫克/天）治疗1周后，血浆葡萄糖和胰岛素水平的升高减弱，血浆GIP水平的升高减少，而tGLP-1水平的升高持续时间更长。得出的结论是，GIP分泌受葡萄糖吸收刺激，tGLP-1分泌受肠道中蔗糖存在的刺激。
