Sun Hai-chen, Qian Xiao-ming, Nie Shi-nan, Wu Xue-hao
Center of Emergency Medicine, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China.
Chin J Traumatol. 2005 Apr;8(2):67-73.
To monitor the systemic gene expression profile in a murine model of lipopolysaccharide-induced acute lung injury.
Acute lung injury was induced by intratracheal injection of lipopolysaccharide in 3 mice. Another 3 normal mice receiving same volume of normal saline were taken as the controls. The comprehensive gene expression profile was monitored by the recently modified long serial analysis of gene expression.
A total of 24,670 tags representing 12,168 transcripts in the control mice and 26,378 tags representing 13,397 transcripts in the mice with lung injury were identified respectively. There were 11 transcripts increasing and 7 transcripts decreasing more than 10 folds in the lipopolysaccharide-treated mice. The most overexpressed genes in the mice with lung injury included serum amyloid A3, metallothionein 2, lipocalin 2, cyclin-dependent kinase inhibitor 1A, lactate dehydrogenase 1, melatonin receptor, S100 calcium-binding protein A9, natriuretic peptide precursor, etc. Mitogen activated protein kinase 3, serum albumin, complement component 1 inhibitor, and ATP synthase were underexpressed in the lung injury mice.
Serial analysis of gene expression provides a molecular characteristic of acute lung injury.
监测脂多糖诱导的小鼠急性肺损伤模型中的全身基因表达谱。
通过气管内注射脂多糖诱导3只小鼠发生急性肺损伤。另外3只接受相同体积生理盐水的正常小鼠作为对照。采用最近改良的基因表达长序列分析方法监测综合基因表达谱。
在对照小鼠中分别鉴定出代表12168个转录本的24670个标签，在肺损伤小鼠中鉴定出代表13397个转录本的26378个标签。在脂多糖处理的小鼠中，有11个转录本增加且7个转录本减少超过10倍。肺损伤小鼠中表达上调最明显的基因包括血清淀粉样蛋白A3、金属硫蛋白2、脂质运载蛋白2、细胞周期蛋白依赖性激酶抑制剂1A、乳酸脱氢酶1、褪黑素受体、S100钙结合蛋白A9、利钠肽前体等。丝裂原活化蛋白激酶3,血清白蛋白、补体成分1抑制剂和ATP合酶在肺损伤小鼠中表达下调。
基因表达序列分析提供了急性肺损伤的分子特征。
