Li Wei-Ying, Lai Bai-Tang, Zhan Xiu-Ping
Beijing Thoracic Tumor and Tuberculosis Research Institute, Beijing 101149, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2004 Dec;25(12):1042-5.
To investigate the relationship between the gene polymorphism of metabolizing enzymes and the genetic susceptibility to lung cancer as well as to study the synergistic effects between smoking and the genes.
A case-control study (case = 217, control = 200) was carried out to compare the frequent distribution of CYP1A1, 2E1, 2D6 and GSTM1 genotypes between the lung cancer group and the control group with a polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) method and to analyze the relationship between these genes and smoking.
GSTM1-null genotype frequency was 58.5% in the lung cancer group and 47.5% in the control group with significant difference (P = 0.02). The frequent distribution of CYP1A1, 2E1, 2D6 genotypes was not significantly different in the two groups (P > 0.05). Synergistic effects were found between smoking and GSTM1 but not between smoking and CYP1A1, 2E1, 2D6.
Smoking and GSTM1-null genotype seemed to be the risk factors of lung cancer. Those who carrying GSTM1-null genotype and smoking cigarettes were prone to suffer from lung cancer to become the high-risk population of the disease.
探讨代谢酶基因多态性与肺癌遗传易感性的关系，并研究吸烟与这些基因之间的协同作用。
采用病例对照研究（病例 = 217，对照 = 200），运用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法比较肺癌组与对照组中CYP1A1、2E1、2D6和GSTM1基因的基因型频率分布，并分析这些基因与吸烟的关系。
肺癌组中GSTM1基因缺失型基因型频率为58.5%，对照组为47.5%，差异有统计学意义（P = 0.02）。两组中CYP1A1、2E1、2D6基因型的频率分布无显著差异（P > 0.05）。吸烟与GSTM1之间存在协同作用，而吸烟与CYP1A1、2E1、2D6之间不存在协同作用。
吸烟和GSTM1基因缺失型基因型可能是肺癌的危险因素。携带GSTM1基因缺失型基因型且吸烟的人群易患肺癌，成为该病的高危人群。
