Gsur A, Haidinger G, Hollaus P, Herbacek I, Madersbacher S, Trieb K, Pridun N, Mohn-Staudner A, Vetter N, Vutuc C, Micksche M
Division of Applied and Experimental Oncology, Institute of Cancer Research, University of Vienna, Austria.
Anticancer Res. 2001 May-Jun;21(3C):2237-42.
Susceptibility to lung cancer may, in part, be determined by interindividual differences in the cytochrome P450-catalysed bioactivation and the glutathione S-transferase-catalysed detoxification of procarcinogens. Therefore a lung cancer case-control study was set up to investigate the association of three polymorphisms of the CYP1A1 gene (CYP1A12A, CYP1A12B, CYP1A14) and GSTM10 genotype with lung cancer risk in Austrian Caucasians. Genomic DNA was isolated from the peripheral blood lymphocytes of 134 male lung cancer patients and 134 age-matched controls with nonmalignant conditions and PCR-based analyses were performed. There was no significant difference in risk between cases and controls, either for the CYP1A12A (OR=1.09, 95%CI=0.46-2.58), CYP1A12B (OR=1.09, 95%CL=0.46-2.58) or for the CYP1A14 polymorphism (OR=0.49, 95%CL=0.20-1.16). The prevalence of the GSTM10 genotype in the lung cancer group (47.8%) was comparable to that found in the control group (49.3%) and also had no effect on lung cancer risk (OR=0.94, 95%CL=0.54-1.57). Further, in a subgroup of male ever-smokers (n=126), no significant influence on the relative risk was found for these polymorphisms. Our results suggest that these investigated polymorphisms can not be considered as genetic susceptibility markers for lung cancer within the Austrian Caucasian population.
肺癌易感性可能部分取决于个体间细胞色素P450催化的生物活化作用以及谷胱甘肽S -转移酶催化的致癌物前体解毒作用的差异。因此，开展了一项肺癌病例对照研究，以调查CYP1A1基因的三种多态性（CYP1A12A、CYP1A12B、CYP1A14）和GSTM10基因型与奥地利白种人肺癌风险的关联。从134例男性肺癌患者和134例年龄匹配的非恶性疾病对照的外周血淋巴细胞中分离基因组DNA，并进行基于PCR的分析。病例组和对照组在CYP1A12A（比值比=1.09，95%可信区间=0.46 - 2.58）、CYP1A12B（比值比=1.09，95%可信区间=0.46 - 2.58）或CYP1A14多态性（比值比=0.49，95%可信区间=0.20 - 1.16）方面的风险均无显著差异。肺癌组中GSTM10基因型的患病率（47.8%）与对照组（49.3%）相当，对肺癌风险也无影响（比值比=0.94，95%可信区间=0.54 - 1.57）。此外，在男性曾经吸烟者亚组（n = 126）中，未发现这些多态性对相对风险有显著影响。我们的结果表明，在奥地利白种人群体中，这些研究的多态性不能被视为肺癌的遗传易感性标志物。
