Inoue Tamotsu, Hibi Kenji, Nakayama Goro, Komatsu Yoshinao, Fukuoka Tomoki, Kodera Yasuhiro, Ito Katsuki, Akiyama Seiji, Nakao Akimasa
Gastroenterological Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8560, Japan.
J Gastroenterol. 2005 Feb;40(2):143-7. doi: 10.1007/s00535-004-1512-9.
It is important to seek the appropriate chemotherapy drugs to effectively eliminate colorectal cancers. To avoid unnecessary medication and uncomfortable side effects, it is important to estimate the chemosensitivity of cancers to 5-fluorouracil (5-FU) before chemotherapy.
We examined thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) gene expressions in 23 colorectal cancers, using quantitative reverse transcription-polymerase chain reaction (RT-PCR). We then evaluated the relationship between TS and DPD gene expression levels and the sensitivity of colorectal cancers to 5-FU, as determined by histoculture drug response assay (HDRA).
A significant increase in the TS expression score was observed in 5-FU-sensitive colorectal cancers (0.57 +/- 0.19) compared to 5-FU-resistant ones (1.16 +/- 0.98; P = 0.029), whereas no significant differences in DPD expression scores were observed in 5-FU-sensitive colorectal cancers (0.86 +/- 1.19) compared to 5-FU-resistant ones (0.56 +/- 1.05; P = 0.603).
TS mRNA may be useful as a predictor of the 5-FU chemosensitivity of colorectal cancers.
寻找合适的化疗药物以有效消除结直肠癌至关重要。为避免不必要的用药及不适的副作用，在化疗前评估癌症对5-氟尿嘧啶（5-FU）的化疗敏感性很重要。
我们采用定量逆转录-聚合酶链反应（RT-PCR）检测了23例结直肠癌中胸苷酸合成酶（TS）和二氢嘧啶脱氢酶（DPD）基因的表达。然后我们评估了TS和DPD基因表达水平与结直肠癌对5-FU敏感性之间的关系，该敏感性通过组织培养药物反应试验（HDRA）确定。
与5-FU耐药的结直肠癌（1.16±0.98；P = 0.029）相比，5-FU敏感的结直肠癌（0.57±0.19）中观察到TS表达评分显著增加，而与5-FU耐药的结直肠癌（0.56±1.05；P = 0.603）相比，5-FU敏感的结直肠癌（0.86±1.19）中DPD表达评分未观察到显著差异。
TS mRNA可能作为结直肠癌5-FU化疗敏感性的预测指标。
