胸苷酸合成酶的表达水平是预测结直肠癌对5-氟尿嘧啶化疗敏感性的良好指标。
Expression level of thymidylate synthase is a good predictor of chemosensitivity to 5-fluorouracil in colorectal cancer.
作者信息
Inoue Tamotsu, Hibi Kenji, Nakayama Goro, Komatsu Yoshinao, Fukuoka Tomoki, Kodera Yasuhiro, Ito Katsuki, Akiyama Seiji, Nakao Akimasa
机构信息
Gastroenterological Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8560, Japan.
出版信息
J Gastroenterol. 2005 Feb;40(2):143-7. doi: 10.1007/s00535-004-1512-9.
BACKGROUND
It is important to seek the appropriate chemotherapy drugs to effectively eliminate colorectal cancers. To avoid unnecessary medication and uncomfortable side effects, it is important to estimate the chemosensitivity of cancers to 5-fluorouracil (5-FU) before chemotherapy.
METHODS
We examined thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) gene expressions in 23 colorectal cancers, using quantitative reverse transcription-polymerase chain reaction (RT-PCR). We then evaluated the relationship between TS and DPD gene expression levels and the sensitivity of colorectal cancers to 5-FU, as determined by histoculture drug response assay (HDRA).
RESULTS
A significant increase in the TS expression score was observed in 5-FU-sensitive colorectal cancers (0.57 +/- 0.19) compared to 5-FU-resistant ones (1.16 +/- 0.98; P = 0.029), whereas no significant differences in DPD expression scores were observed in 5-FU-sensitive colorectal cancers (0.86 +/- 1.19) compared to 5-FU-resistant ones (0.56 +/- 1.05; P = 0.603).
CONCLUSIONS
TS mRNA may be useful as a predictor of the 5-FU chemosensitivity of colorectal cancers.
背景
寻找合适的化疗药物以有效消除结直肠癌至关重要。为避免不必要的用药及不适的副作用，在化疗前评估癌症对5-氟尿嘧啶（5-FU）的化疗敏感性很重要。
方法
我们采用定量逆转录-聚合酶链反应（RT-PCR）检测了23例结直肠癌中胸苷酸合成酶（TS）和二氢嘧啶脱氢酶（DPD）基因的表达。然后我们评估了TS和DPD基因表达水平与结直肠癌对5-FU敏感性之间的关系，该敏感性通过组织培养药物反应试验（HDRA）确定。
结果
与5-FU耐药的结直肠癌（1.16±0.98；P = 0.029）相比，5-FU敏感的结直肠癌（0.57±0.19）中观察到TS表达评分显著增加，而与5-FU耐药的结直肠癌（0.56±1.05；P = 0.603）相比，5-FU敏感的结直肠癌（0.86±1.19）中DPD表达评分未观察到显著差异。
结论
TS mRNA可能作为结直肠癌5-FU化疗敏感性的预测指标。
Zotero 插件