Shoji Y, Itoh T, Kagiyama N
Central Institute for Experimental Animals, Kanagawa, Japan.
Jikken Dobutsu. 1992 Apr;41(2):231-4. doi: 10.1538/expanim1978.41.2_231.
CAR bacillus propagated successfully in an artificial medium, and the number of CAR bacillus was about 30 times the original number after 8 days of cultivation. The medium consisted of Eagle's minimum essential medium supplemented with 10% fetal calf serum and 20% hamster tracheal organ culture soup. By intranasal inoculation to mice, two strains of the CAR bacillus passaged 5 and 6 times in this artificial medium produced the same lung lesions as natural CAR bacillus infection.
CAR杆菌在人工培养基中成功繁殖，培养8天后CAR杆菌数量约为原来的30倍。培养基由补充了10%胎牛血清和20%仓鼠气管器官培养液的伊格尔氏最低限度基本培养基组成。通过对小鼠进行鼻内接种，在这种人工培养基中传代5次和6次的两株CAR杆菌产生了与天然CAR杆菌感染相同的肺部病变。
