胍基展示的构象稳定性和几何结构对β-肽进入细胞的影响。

Effects of conformational stability and geometry of guanidinium display on cell entry by beta-peptides.

作者信息

Potocky Terra B, Menon Anant K, Gellman Samuel H

机构信息

Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.

出版信息

J Am Chem Soc. 2005 Mar 23;127(11):3686-7. doi: 10.1021/ja042566j.

DOI:10.1021/ja042566j

PMID:15771489

Abstract

We have used our ability to control beta-peptide secondary structure in order to explore the effects of conformational stability and geometry of guanidinium display on cell entry. Both of these factors affect the rate and relative amount of beta-peptide accumulation in the cytoplasm and nucleus of live HeLa cells. These beta-peptides do not show significant differences in cell surface binding, implying that structure and guanidinium display are important in a later step in cell entry than initial surface binding.

摘要

我们利用自身控制β-肽二级结构的能力，来探究胍基展示的构象稳定性和几何形状对细胞摄取的影响。这两个因素都会影响活的HeLa细胞细胞质和细胞核中β-肽积累的速率和相对量。这些β-肽在细胞表面结合方面未显示出显著差异，这意味着结构和胍基展示在细胞摄取过程中比初始表面结合更靠后的步骤中起重要作用。