Chen Bing-Ya, Ye Da-Feng, Xie Xing, Chen Huai-Zeng, Lü Wei-Guo
Women's Hospital, school of medicine, Zhejiang University, Hangzhou 310006, China.
Zhonghua Zhong Liu Za Zhi. 2005 Jan;27(1):33-7.
To study the expressions of VEGF/VEGFRs and activation of STATs in ovarian epithelial carcinoma, and to elucidate direct effect of VEGF on ovarian carcinoma cells.
Tissue samples from 42 women with primary ovarian epithelial carcinoma (OVCA), 29 with begnin ovarian tumor (OVBT) and 11 with normal ovarian tissue (NOV) were collected. LSAB immunohistochemical staining was used to determine the expression of VEGF, VEGFR1, VEGFR2 and activated STATS (P-STAT1, P-STAT3, P-STAT5, P-STAT6) proteins.
(1) Semi-quantitative scoring showed that VEGF expression in OVCA was significantly higher than that in OVBT and NOV (P < 0.01). Expressions of VEGFR1 and VEGFR2 were significantly elevated in OVCA, including tumor cells and stromal vascular endothelial cells (P < 0.01, compared with OVBT and NOV). There was no difference in VEGFRs expressions between OVBT and NOV. (2) In OVCA, tumor cells and endothelial cells expressed P-STAT3 and P-STAT5 at significantly higher levels than those in OVBT and NOV (P = 0.000). The staining of P-STAT1 and P-STAT6 was weak with no significant differences among OVCA, OVBT and NOV. (3) Expressions of VEGFR1 and VEGFR2 in endothelial cells were significantly correlated with P-STAT5 and P-STAT3, respectively (P = 0.006 and 0.001). In cancer cells, VEGF, VEGFR1 and VEGFR2 were all significantly correlated with P-STAT3 and P-STAT5 (P = 0.000), but not with P-STAT1 or P-STAT6.
VEGF affects ovarian carcinoma cells via VEGFRs, and STATs probably participate in intracellular signaling of VEGF.
研究血管内皮生长因子（VEGF）/血管内皮生长因子受体（VEGFRs）的表达及信号转导和转录激活因子（STATs）的激活情况在卵巢上皮癌中的作用，阐明VEGF对卵巢癌细胞的直接作用。
收集42例原发性卵巢上皮癌（OVCA）患者、29例良性卵巢肿瘤（OVBT）患者及11例正常卵巢组织（NOV）患者的组织样本。采用链霉菌抗生物素蛋白-过氧化物酶连接法（LSAB）免疫组化染色检测VEGF、VEGFR1、VEGFR2及活化的STATs（P-STAT1、P-STAT3、P-STAT5、P-STAT6）蛋白的表达。
（1）半定量评分显示，OVCA中VEGF的表达显著高于OVBT和NOV（P<0.01）。OVCA中VEGFR1和VEGFR2的表达显著升高，包括肿瘤细胞和基质血管内皮细胞（与OVBT和NOV相比，P<0.01）。OVBT和NOV之间VEGFRs的表达无差异。（2）在OVCA中，肿瘤细胞和内皮细胞中P-STAT3和P-STAT5的表达水平显著高于OVBT和NOV（P=0.000）。P-STAT1和P-STAT6的染色较弱，OVCA、OVBT和NOV之间无显著差异。（3）内皮细胞中VEGFR1和VEGFR2的表达分别与P-STAT5和P-STAT3显著相关（P=0.006和0.001）。在癌细胞中，VEGF、VEGFR1和VEGFR2均与P-STAT3和P-STAT5显著相关（P=0.000），但与P-STAT1或P-STAT6无关。
VEGF通过VEGFRs影响卵巢癌细胞，STATs可能参与VEGF的细胞内信号传导。
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