Brustmann Hermann, Naudé Susanna
Department of Pathology, Landeskrankenhaus, Moedling/ Vienna, A-2340, Austria.
Gynecol Oncol. 2002 Jan;84(1):47-52. doi: 10.1006/gyno.2001.6467.
The aim of this study was to investigate the expression of vascular endothelial growth factor (VEGF) by neoplastic cells in serous ovarian cystadenocarcinoma in relation to proliferation as determined by mitotic activity and to FIGO stage.
Formalin-fixed, paraffin-embedded archival tissue from 10 benign serous cystadenomas and 45 serous carcinomas was immunostained with a polyclonal antibody to VEGF (Biogenex) utilizing the LSAB kit, alkaline phosphatase (Dako). Positivity was scored as (0) for no, (+) for weak, and (++) for strong staining. Mitotic activity was determined on hematoxylin and eosin stained sections as mitotic figures per 10 high power fields; for carcinomas, three mitotic activity groups (MAG) were established (0-9 = I [N = 13], 10-24 = II [N = 18], >24 = III [N = 14]). The data were analyzed by Fisher's exact test.
In cystadenomas, focal and weak (+) VEGF expression was found in six cases; mitoses were rare. In carcinomas, no significant difference of (+) and (++) staining intensity was found between MAG I and II (P = 0.6239). In MAG II, focal (++) staining was frequently observed on a background of (+). (++) staining was significantly more frequent in MAG III compared with MAG II (P = 0.0027). Diffuse (++) was more common in MAG III than in MAG II (P = 0.0062). FIGO III cases displayed more frequently a diffuse (++) staining than the FIGO I and FIGO II cases taken together (P = 0.0017).
VEGF expression as determined by immunohistochemistry is related to high mitotic activity and high FIGO stage in serous ovarian cystadenocarcinomas. VEGF expression seems to be an interesting indicator of neoplastic proliferation and may offer therapeutic options for these cancers.
本研究旨在调查浆液性卵巢囊腺癌中肿瘤细胞血管内皮生长因子（VEGF）的表达情况，并将其与通过有丝分裂活性确定的增殖情况以及国际妇产科联盟（FIGO）分期相关联。
采用莱卡生物系统碱性磷酸酶（Dako）LSAB试剂盒及抗VEGF多克隆抗体（Biogenex），对10例良性浆液性囊腺瘤和45例浆液性癌的福尔马林固定、石蜡包埋存档组织进行免疫染色。阳性结果按无染色记为（0）、弱染色记为（+）、强染色记为（++）进行评分。在苏木精和伊红染色切片上确定有丝分裂活性，以每10个高倍视野中的有丝分裂象计数；对于癌，建立了三个有丝分裂活性组（MAG）（0 - 9 = I组 [N = 13]，10 - 24 = II组 [N = 18]，>24 = III组 [N = 14]）。数据采用Fisher精确检验进行分析。
在囊腺瘤中，6例发现局灶性且弱阳性（+）VEGF表达；有丝分裂罕见。在癌中，MAG I组和II组之间（+）和（++）染色强度无显著差异（P = 0.6239）。在MAG II组中，常观察到在（+）背景上有局灶性（++）染色。与MAG II组相比，MAG III组中（++）染色明显更频繁（P = 0.0027）。弥漫性（++）在MAG III组中比在MAG II组中更常见（P = 0.0062）。FIGO III期病例比FIGO I期和FIGO II期病例合计更频繁地显示弥漫性（++）染色（P = 0.0017）。
免疫组化确定的VEGF表达与浆液性卵巢囊腺癌中的高有丝分裂活性和高FIGO分期相关。VEGF表达似乎是肿瘤增殖的一个有趣指标，并可能为这些癌症提供治疗选择。
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