Pang Jian-Xin, Ma Ren-Qiang, Liu Lan-Mei, Jiang Yi-Ping, Sun Li-Sha
Department of Pharmacology, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Mar;25(3):325-8.
To observe the cytotoxic effects in vitro and antitumor effects in vivo of total alkaloid of Macleaya cordata.
MTT assay was used to assess the proliferation of Hep3B and H22 cells in vitro treated with the alkloid, and the inhibitory effects of the alkaloid on H22 and S180 tumors were observed in mice with subcutaneous inoculation of the tumor cells.
The total alkaloid significantly inhibited the proliferation of human Hep3B cells and murine H22 cells in a dose-dependent in vitro. IC(50) of the alkloid in 3 repeated experiments was 3.04, 3.98 and 2.98 mug/ml respectively in Hep3B cells, and was 2.89, 2.21 and 2.34 mug/ml in H22 cells. In the tumor-bearing mice, the alkaloid inhibited the development of H22 tumor and prolonged the survival of S180 tumor-bearing mice. At the daily dose of 1, 2, and 4 mg/kg.b.w (i.p.) and 4 mg/kg.b.w. (i.g.) for 10 days, the inhibition rates of the alkaloid on H22 tumor in 3 repeated experiments were 18.6%, 35.1%, 44.9% and 7.9% respectively, and the rates of survival prolongation of the tumor-bearing mice were 24.8%, 48.9%, and 52.7%, respectively.
The alkloid possesses antitumor effects both in vitro and in vivo.
观察博落回总生物碱的体外细胞毒性作用及体内抗肿瘤作用。
采用MTT法检测博落回总生物碱对体外培养的Hep3B和H22细胞增殖的影响；观察博落回总生物碱对皮下接种肿瘤细胞的小鼠H22和S180肿瘤的抑制作用。
博落回总生物碱在体外可显著抑制人Hep3B细胞和小鼠H22细胞的增殖，且呈剂量依赖性。在3次重复实验中，博落回总生物碱对Hep3B细胞的IC(50)分别为3.04、3.98和2.98μg/ml，对H22细胞的IC(50)分别为2.89、2.21和2.34μg/ml。在荷瘤小鼠中，博落回总生物碱可抑制H22肿瘤生长，延长S180荷瘤小鼠的生存期。每日腹腔注射1、2和4mg/kg.b.w.及灌胃4mg/kg.b.w.给药10天，在3次重复实验中，博落回总生物碱对H22肿瘤的抑制率分别为18.6%、35.1%、44.9%和7.9%，荷瘤小鼠的生存期延长率分别为24.8%、48.9%和52.7%。
博落回总生物碱具有体外和体内抗肿瘤作用。
