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[凝固酶阴性葡萄球菌对生物材料的黏附依赖性以及icaADBC操纵子存在时PIA多糖的合成]

[Adhesion dependence of coagulase-negative staphylococci to biomaterials and PIA polysaccharide synthesis on icaADBC operon presence].

作者信息

Bartoszewicz Marzenna, Nowicka Joanna, Janikowska Ewa, Przondo-Mordarska Anna

机构信息

Zakład Mikrobiologii Akademii Medycznej we Wrocławiu.

出版信息

Med Dosw Mikrobiol. 2004;56(3):225-30.

Abstract

Both Staphylococcus epidermidis and Staphylococcus haemolyticus are important causes of infections associated with catheters and other medical devices. This infections result in significant morbidity, mortality and economic cost. It has recently been shown that not only S. epidermidis but also S. haemolyticus can produce slime and carries the ica operon responsible for and slime production. In the operon, coexpression of icaA and icaD is required for full slime synthesis. This study is focused on detecting icaA and icaD genes in S. haemolyticus and comparison of these two species. It turned out that strain representatives within the same species behave very differently and a single tested strain from each species is unlikely to be representative of the species as a whole. Contrary to S. epidermidis, S. haemolyticus strain appeared to carry no icaA-like and icaD-like genes, but was able to form biofilm in vitro.

摘要

表皮葡萄球菌和溶血葡萄球菌都是与导管及其他医疗设备相关感染的重要病因。这类感染会导致显著的发病率、死亡率和经济成本。最近研究表明,不仅表皮葡萄球菌,溶血葡萄球菌也能产生黏液,并携带负责黏液产生的ica操纵子。在该操纵子中,icaA和icaD的共表达是完全合成黏液所必需的。本研究聚焦于检测溶血葡萄球菌中的icaA和icaD基因,并对这两个菌种进行比较。结果发现,同一菌种内的菌株代表表现差异很大,每个菌种仅测试一个菌株不太可能代表整个菌种。与表皮葡萄球菌相反,溶血葡萄球菌菌株似乎不携带icaA样和icaD样基因,但能够在体外形成生物膜。

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